Anti-5HT1D Receptor (HTR1D) (extracellular) Antibody

5-Hydroxytryptamine receptor 1D, Serotonin receptor 1D, 5HT1D-alpha, HTR1DA
    Cat #: ASR-023
    Alternative Name 5-Hydroxytryptamine receptor 1D, Serotonin receptor 1D, 5HT1D-alpha, HTR1DA
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, r
      • Peptide (C)EASNRSLNATGAWD, corresponding to amino acid residues 14-27 of rat 5-hydroxytryptamine receptor 1D (Accession P28565). Extracellular, N-terminus.
        Anti-5HT1D Receptor (HTR1D) (extracellular) Antibody
    Accession (Uniprot) Number P28565
    Gene ID 25323
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human - 13/14 amino acid residues identical.
    RRID AB_10917438.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
      • Anti-5HT1D Receptor (HTR1D) (extracellular) Antibody
        Western blot analysis of rat prostate (lanes 1 and 3) and PC-3 cell (lanes 2 and 4) lysates:
        1,3. Anti-5HT1D Receptor (HTR1D) (extracellular) Antibody (#ASR-023), (1:200).
        2,4. Anti-5HT1D Receptor (HTR1D) (extracellular) Antibody, preincubated with the control peptide antigen.
    References
      • Serotonin (5-hydroxytryptamine, 5-HT) plays unequivocally a most important role in the normal physiology amongst higher eukaryotes, as do most neurotransmitters. The physiological roles of serotonin and its receptors range from appetite, regulation of the circadian rhythm, sexual behavior, memory and nociception. Serotonin is also involved in migraine and some psychiatric diseases such as depression anxiety and aggression1. Serotonin and its receptors also have an influence on the cardiovascular, pulmonary, gastrointestinal (GI) and genitourinary systems2.

        To date, some fifteen serotonin receptors have been cloned and grouped into seven families (5-HT1-5-HT7) based on their signaling mechanisms. The 5-HT1 receptor class comprises 5 different receptors with ranging homology. Apart from 5-HT3, all serotonin receptors belong to the G-protein coupled receptor superfamily and therefore span the membrane seven times3.

        Members of the 5-HT1 receptor class all couple Gi/0 thereby inhibiting adenylate cyclase.

        In the central nervous system, 5-HT1D is expressed in the basal ganglia, hippocampus and cortex in the rat and in the basal ganglia, midbrain and spinal cord in the human. In serotonin neurons, 5-HT1D receptors are autoreceptors thereby inhibiting the release of 5-HT. In GABA, ACh, and glutamate neurons they are heteroreceptors where they control the release of these neurotransmitters4,5.

        The serotonin system plays an undeniable role in the perception and processing of pain. Indeed, many migraine sufferers are prescribed triptan-based drugs which activate 5-HT1B and 5-HT1D receptors2.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IF- Immunofluorescence, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 18/08/2019

    Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the rat 5-Hydroxytryptamine receptor 1D. Anti-5HT1D Receptor (HTR1D) (extracellular) Antibody (#ASR-023) can be used in western blot analysis. It has been designed to recognize 5HT1D receptor from human, rat and mouse samples.

    For research purposes only, not for human use