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Anti-5HT2B Receptor (HTR2B) (extracellular)-ATTO Fluor-488 Antibody

5-Hydroxytryptamine receptor 2B, Serotonin receptor 2B, 5-HT-2B, 5-HT2F, Stomach fundus serotonin receptor
Cat #: ASR-035-AG
Alternative Name 5-Hydroxytryptamine receptor 2B, Serotonin receptor 2B, 5-HT-2B, 5-HT2F, Stomach fundus serotonin receptor
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)KMSEQSTISEHILQK, corresponding to amino acid residues 6-20 of rat 5-Hydroxytryptamine receptor 2B (Accession P30994). Extracellular, N-terminus.
Accession (Uniprot) Number P30994
Gene ID 28581
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - identical, human - 11/15 amino acid residues identical.
RRID AB_2756795.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Label ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 – 515 nm range. This label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #ASR-035), and immunohistochemistry (labeled antibody).
Applications: ih
May also work in: ic*, lci*
Immunohistochemistry
  • Expression of 5-Hydroxytryptamine receptor 2B in mouse cerebellum
    Expression of 5-Hydroxytryptamine receptor 2B in mouse cerebellum
    Immunohistochemical staining of mouse cerebellum using Anti-5HT2B Receptor (HTR2B) (extracellular)-ATTO Fluor-488 Antibody (#ASR-035-AG), (1:80). A. 5-HT2B staining (green) appears in neurons of the Purkinje layer (vertical arrows) and in the molecular layer (horizontal arrow). B. Nuclear staining using DAPI as the counterstaining (blue). C. Merged image of panels A and B.
References
  1. Hutcheson, J.D. et al. (2011) Pharmacol. Ther. 132, 146.
  2. Fitzgerald, L.W. et al. (2000) Mol. Pharmacol. 57, 75.
  3. Nelson, P.M. et al. (2012) Exp. Diabetes Res. 2012, 11.
  4. Schmuck, K. et al. (1994) FEBS Lett. 342, 85.
  5. Nebigil, C.G. et al. (2003) Circulation 107, 3223.
Scientific background

5-HT is involved in a variety of physiological and biological processes. In the brain, 5-HT has been found to affect sleep, mood, appetite, anxiety, aggression, perception, pain, and cognition1. Signaling of 5-HT is mediated by receptors that are located on the cell membrane of neurons and most other cells in the body. The 5-HT2 family consists of three G-protein coupled receptors (GPCRs): 5-HT2A5-HT2B, and 5-HT2C2. Like other members, they are transmembrane proteins consisting of seven membrane-spanning α-helical segments with an extracellular N-terminus and an intracellular C-terminus. The binding of 5-HT to one of its receptors is thought to elicit a conformational change that activates associated heterotrimeric G proteins and recruits other downstream signaling/scaffolding molecules, such as GPCR kinases and β-arrestins.

5-HT2B receptors have been found to be present in both rodent and human tissues, particularly in the cardiovascular system, gastrointestinal tract, bone, and central nervous system2. Only low expression levels are found in the brain and blood. The highest expression levels are found in the stomach fundus3.

Over-expression of 5-HT2B receptors in hearts of transgenic mice results in cardiac hypertrophy and decreased ventricular function due to enhanced extracellular matrix (ECM) and remodeling. Likewise, genetic deletion of 5-HT2B receptors was shown to lead to ventricular dilation and incomplete cardiac development5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Anti-5HT2B Receptor (HTR2B) (extracellular) Antibody (#ASR-035) is a highly specific antibody directed against an epitope of the rat 5-HT-2B receptor. The antibody can be used in western blot and immunohistochemistry applications. It recognizes an extracellular epitope and can potentially detect 5HT2B receptor in living cells. It has been designed to recognize 5-hydroxytryptamine receptor 2B from rat, mouse and human samples.

Anti-5HT2B Receptor (HTR2B) (extracellular)-ATTO Fluor-488 Antibody (#ASR-035-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-5HT2B Receptor (HTR2B) (extracellular)-ATTO Fluor-488 Antibody is specially suited to experiments requiring simultaneous labeling of different markers.

For research purposes only, not for human use
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