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Anti-5HT1B Receptor (HTR1B) (extracellular)-ATTO Fluor-488 Antibody

5-Hydroxytryptamine receptor 1B, Serotonin receptor 1B, Serotonin 1D beta receptor, 5HT1D-beta, HTR1DB, HTR1D2
Cat #: ASR-022-AG
Alternative Name 5-Hydroxytryptamine receptor 1B, Serotonin receptor 1B, Serotonin 1D beta receptor, 5HT1D-beta, HTR1DB, HTR1D2
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide CSAKDYIYQDSISLPWK, corresponding to amino acid residues 33-49 of human 5-Hydroxytryptamine receptor 1B (Accession P28222). Extracellular, N-terminus.
Accession (Uniprot) Number P28222
Gene ID 3351
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat - 15/17 amino acid residues identical; mouse - 14/17 amino acid residues identical.
RRID AB_2756794.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Label ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the dye fluorescein isothiocyanate (FITC) and can be used with filters used to detect FITC.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #ASR-022), and direct flow cytometry (labeled antibody).
Applications: if, ih
May also work in: ic*, lci*
Immunohistochemistry
  • Expression of 5-Hydroxytryptamine receptor 1B in rat and mouse brain
    Expression of 5-Hydroxytryptamine receptor 1B in rat and mouse brain
    Immunohistochemical staining of rat and mouse brain sections using Anti-5HT1B Receptor (HTR1B) (extracellular)-ATTO Fluor-488 Antibody (#ASR-022-AG), (1:80). A. 5-HT1B receptor staining (green) in rat hippocampal CA1 region is detected near the pyramidal layer (P) and in apical dendrites (arrows). B. In mouse hippocampal CA1 region, 5-HT1B receptor staining (green) appears in the pyramidal layer (P) and in apical dendrites. Nuclei are stained using DAPI (blue).
References
  1. Sari, Y. (2004) Neurosi. Biobehav. Rev. 28, 565.
  2. Berger, M. et al. (2009) Annu. Rev. Med. 60, 355.
  3. 5-Hydroxytryptamine receptors on IUPHAR.
Scientific background

Serotonin (5-hydroxytryptamine, 5-HT) plays unequivocally a most important role in the normal physiology amongst higher eukaryotes, as do most neurotransmitters. The physiological roles of serotonin and its receptors range from appetite, regulation of the circadian rhythm, sexual behavior, memory and nociception. Serotonin is also involved in migraine and some psychiatric diseases such as depression anxiety and aggression1. Serotonin and its receptors also have an influence on the cardiovascular, pulmonary, gastrointestinal (GI) and genitourinary systems2.

To date, some fifteen serotonin receptors have been cloned and grouped into seven families (5-HT1-5-HT7) based on their signaling mechanisms. The 5-HT1 receptor class comprises 5 different receptors with ranging homology. Apart from 5-HT3, all serotonin receptors belong to the G-protein coupled receptor superfamily and therefore span the membrane seven times3.

The 5-hydroxytryptamine receptor 1B (5-HT1B) is involved in several physiological behaviors and psychiatric diseases, including locomotor activity, drug abuse reinforcement, migraine, anxiety states and aggressive behaviors1.

Pharmacological studies suggest that 5-HT1B is expressed by both serotonergic and non-serotonergic neurons. In the nervous system, this receptor is located in the axon terminal of the plasma membrane of unmyelinated axons. It is predominantly localized on pre-synaptic membranes. Signal transduction studies of the receptor indicate that this receptor is coupled negatively to adenylyl cyclase as its activation induces a decrease in adenylyl cyclase activity1.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Anti-5HT1B Receptor (HTR1B) (extracellular) Antibody (#ASR-022) is a highly specific antibody directed against an epitope of the human 5-Hydroxytryptamine receptor 1B. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize 5HT1B receptor from human, rat, and mouse samples.

Anti-5HT1B Receptor (HTR1B) (extracellular)-ATTO Fluor-488 Antibody (#ASR-022-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well-known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-5HT1B Receptor (HTR1B) (extracellular)-ATTO Fluor-488 Antibody is specially suited to experiments requiring simultaneous labeling of different markers.

For research purposes only, not for human use
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