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Anti-Adenosine A2A Receptor Antibody

ADORA2A, A2AAR, Adenosine A2aR

Cat #: AAR-002
Alternative Name ADORA2A, A2AAR, Adenosine A2aR
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)RQLKQMESQPLPGER, corresponding to amino acid residues 201-215 of mouse adenosine A2A receptor (Accession Q60613). 3rd intracellular loop.
Accession (Uniprot) Number Q60613
Gene ID 11540
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, human, and canis – identical.
RRID AB_2039707.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.75 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ih, wb
May also work in: ifc*, ip*
Western blot
  • Western blot analysis of rat aortic endothelial cells (lanes 1, 3), rat brain (lanes 2, 4) and Jurkat (lanes 5, 6) lysates:
    Western blot analysis of rat aortic endothelial cells (lanes 1, 3), rat brain (lanes 2, 4) and Jurkat (lanes 5, 6) lysates:
    1,2,5. Anti-Adenosine A2A Receptor Antibody (#AAR-002), (1:200).
    3,4,6. Anti-Adenosine A2A Receptor Antibody, preincubated with Adenosine A2A Receptor Blocking Peptide (#BLP-AR002).
Immunohistochemistry
  • Expression of Adenosine A2A Receptor in mouse diagonal band
    Expression of Adenosine A2A Receptor in mouse diagonal band
    Immunohistochemical staining of mouse diagonal band using Anti-Adenosine A2A Receptor Antibody (#AAR-002). A2aR (green) appears in the broca of individual neurons (arrows) and in neuropil (asterisk). DAPI is used as the counterstain.
  • Expression of Adenosine A2A Receptor in rat lung
    Expression of Adenosine A2A Receptor in rat lung
    Immunohistochemical staining of paraffin emedded rat lung sections using Anti-Adenosine A2A Receptor Antibody (#AAR-002), (1:50). A2aR is expressed in the respiratory epithelium of the bronchioli (Br). Note that smooth muscle and endothelium in blood vessels are negative. Hematoxilin is used as the counterstain.
Immunocytochemistry
References
  1. Okusa, M.D. (2002) Am. J. Physiol. 282, F10.
  2. Fredholm, B.B. et al. (2001) Pharmacol. Rev. 53, 527.
  3. Nakata, H. (1989) J. Biol. Chem. 264, 16545.
  4. Cunha, R.A. (2001) Neurochem. Int. 38, 107.
  5. Fredholm, B.B. et al. (2000) Naunyn-Schmiedebergs Arch. Pharmacol. 362, 364.
  6. Canals, M. et al. (2005) J. Neurochem. 92, 337.
  7. Ohta, A. and Sitkovsky, M. (2001) Nature 414, 916.
  8. Ohta, A. et al. (2006) Proc. Natl. Acad. Sci. 103, 13132.
Scientific background

Adenosine is an endogenous nucleoside generated locally in tissues under conditions of hypoxia, ischemia, or inflammation. It modulates a variety of physiological functions in many tissues including the brain and heart.1,2 Adenosine exerts its actions via four specific adenosine receptors (also named P1 purinergic receptors): Adenosine A1 Receptor (A1AR), Adenosine A2A Receptor (A2AAR), Adenosine A2B Receptor (A2BAR), and Adenosine A3 Receptor (A3AR). All are integral membrane proteins and are members of the G protein-coupled receptor superfamily. They share a common structure of seven putative transmembrane domains, an extracellular -NH2 terminus, cytoplasmic -COOH terminus, and a third intracellular loop important for binding G proteins.1-3 The adenosine receptors can be distinguished on the basis of their differential selectivity for adenosine analogs.1-3

Adenosine receptors control neurotransmitter release through the facilitatory A2AAR and the inhibitory A1AR.4 A2AAR and A1AR are the major adenosine receptor subtypes expressed in the central nervous system (CNS). A2AAR is mainly expressed in the striatum on GABAergic striatopallidal neurons, while A1AR is widely distributed throughout the CNS.5,6

A2AAR was suggested to play a critical role in attenuation of systemic inflammatory responses and prevention of extensive tissue damage.7 It was suggested that extracellular adenosine that accumulates in inflamed and damaged tissue may activate the A2AAR expressed in immune cells leading to termination/inhibition of the immune response.7 It was further suggested that this same mechanism may protect tumors from antitumor T cells through an immunosuppressive signal generated by the activation of A2AAR on T cells by extracellular adenosine produced from hypoxic cancerous tissues.8

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Anti-Adenosine A2A Receptor Antibody (#AAR-002) is a highly specific antibody directed against an epitope of mouse protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize A2aR from human, rat, and mouse samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Western blot citations
  1. Human ovarian cancer A1780 and HEY cell lysates.
    Sureechatchaiyan, P. et al. (2018) Purinergic Signal. 14, 395.
  2. Human skin lysate.
    Andres, R.M. et al. (2017) J. Invest. Dermatol. 137, 123.
  3. Rat lung lysate.
    Densmore, J.C. et al. (2017) Exp. Lung Res. 43, 38.
  4. Mouse brain lysate.
    Ingwersen, J. et al. (2016) J. Neuroinflammation 13, 1.
Immunohistochemistry citations
  1. Rat Cochlea sections (1:50).
    Han, B.R.X. et al. (2019) Cells 8, 877.
Immunocytochemistry citations
  1. Rat neurospheres.
    Benito-Munoz, M. et al. (2016) Glia 64, 1465.
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