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Anti-Adenosine A2B Receptor (extracellular)-FITC Antibody

ADORA2b, A2BAR, Adenosine A2bR
Cat #: AAR-003-F
Alternative Name ADORA2b, A2BAR, Adenosine A2bR
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m
May also work in: r*
Immunogen
  • Peptide KDSATNN(S)TEPWDGTTNESC, corresponding to amino acid residues 147-166 of human A2BAR (Accession P29275). 2nd extracellular loop.
Accession (Uniprot) Number P29275
Gene ID 136
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat - 14/19 amino acid residues identical; mouse - 13/19 amino acid residues identical
RRID AB_2756535.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Label Fluorescein isothiocyanate (FITC).
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #AAR-003), and direct flow cytometry (labeled antibody).
Applications: fc
May also work in: ic*, lci*
Direct flow cytometry
  • Cell surface detection of ADORA2b in live intact human THP-1 monocytic leukemia cells:
    Cell surface detection of ADORA2b in live intact human THP-1 monocytic leukemia cells:
    ___ Cells alone.
    ___ Cells + Rabbit IgG isotype control-FITC.
    ___ Cells + Adenosine A2B Receptor (extracellular)-FITC Antibody (#AAR-003-F), (5 µg).
  • Cell surface detection of ADORA2b in live intact mouse J774 macrophage cells:
    Cell surface detection of ADORA2b in live intact mouse J774 macrophage cells:
    ___ Cells alone.
    ___ Cells + Rabbit IgG isotype control-FITC.
    ___ Cells + Adenosine A2B Receptor (extracellular)-FITC Antibody (#AAR-003-F), (5 µg).
References
  1. Peeters, M.C. et al. (2011) FASEB J. 25, 632.
  2. Sun, Y. and Huang, P. (2016) Front. Chem. 4, 37.
Scientific background

Extracellular adenosine is a ubiquitous signaling molecule that modulates a wide array of biological processes. There are currently four known sub-types of adenosine receptors: A1, A2A, A2B and A3.
 
The adenosine A2B receptor is a typical class A, G protein-coupled receptor with seven transmembrane segments, an extracellular N-terminus and an intracellular C-terminus. Adenosine A2BR causes an increase in intracellular cAMP levels by coupling mainly to Gs proteins, resulting in the activation of adenylyl cyclase1 and subsequent activation of PKA and other cAMP effectors such as Epac. In addition, the A2BR couples to Gq and activates the PLC pathway that mediates several crucial functions of A2BR. A2BR also couples to the MAPK and arachidonic acid signaling pathways and regulates membrane ion channels probably through G-protein βγ subunits.
 
Multiple molecules that bind to A2BR have been identified in an effort to understand the receptor’s function and regulation. NFκB1/p105 is a member of the NFκB family of proteins that perform regulatory functions in diverse biological processes such as inflammation and cell survival and differentiation, as well as in various diseases. Interestingly, Although A2BR activation by adenosine produces pro-inflammatory effects the receptor also binds to specific sites on p105, prevents the poly-ubiquitination and degradation of the p105 protein and inhibits NFκB activation thus reducing inflammation.
 
A2BR is highly expressed in various types of tumor cells or tissues and promotes tumor-cell proliferation. A2BR was found to be overexpressed in colorectal carcinoma cells and tissues, and inhibition of A2BR blocks the proliferation of colon cancer cells. A2BR has been implicated in the pathophysiology of vascular diseases, renal disease and diabetes2.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Anti-Adenosine A2B Receptor (extracellular) Antibody (#AAR-003) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, live cell imaging, and indirect flow cytometry applications. It has been designed to recognize A2BAR from human, rat, and mouse samples.

Anti-Adenosine A2B Receptor (extracellular)-FITC Antibody (#AAR-003-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody can be used in immunofluorescent applications such as direct flow cytometry using live cells.

For research purposes only, not for human use
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