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Anti-ACE2 (extracellular) Antibody

Angiotensin-Converting Enzyme 2, ACE-Related Carboxypeptidase, Angiotensin-converting enzyme homolog, ACEH, Metalloprotease MPROT15

Cat #: AAC-012
Alternative Name Angiotensin-Converting Enzyme 2, ACE-Related Carboxypeptidase, Angiotensin-converting enzyme homolog, ACEH, Metalloprotease MPROT15
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)EDYGDYWRGDYEVNGVD, corresponding to amino acid residues 197 - 213 of human ACE2 (Accession Q9BYF1). Extracellular, N-term.
Accession (Uniprot) Number Q9BYF1
Gene ID 59272
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat –  14 out of 17 amino acid residues identical, mouse –  13 out of 17 amino acid residues identical
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Preadsorption Control 1 µg peptide per 1 µg antibody
Standard quality control of each lot Western blot analysis.
Applications: ifc, ih, lci, wb
May also work in: ic*, ip*
Western blot
  • Western blot analysis of rat small intestine lysate (lanes 1 and 4), mouse lung lysate (lanes 2 and 5 ) and mouse kidney lysate (lanes 3 and 6):
    Western blot analysis of rat small intestine lysate (lanes 1 and 4), mouse lung lysate (lanes 2 and 5 ) and mouse kidney lysate (lanes 3 and 6):
    1-3. Anti-ACE2 (extracellular) Antibody (#AAC-012), (1:200).
    4-6. Anti-ACE2 (extracellular) Antibody, preincubated with ACE2 (extracellular) Blocking Peptide (BLP-AC012).
  • Western blot analysis of human HepG2 hepatoma cell line lysate (lanes 1 and 3) and human THP-1 monocytic leukemia cell line lysate (lanes 2 and 4):
    Western blot analysis of human HepG2 hepatoma cell line lysate (lanes 1 and 3) and human THP-1 monocytic leukemia cell line lysate (lanes 2 and 4):
    1-2. Anti-ACE2 (extracellular) Antibody (#AAC-012), (1:200).
    3-4. Anti-ACE2 (extracellular) Antibody, preincubated with ACE2 (extracellular) Blocking Peptide (BLP-AC012).
Immunohistochemistry
  • Expression of ACE2 in mouse thalamus.
    Expression of ACE2 in mouse thalamus.
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-ACE2 (extracellular) Antibody (#AAC-012), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. ACE2 immunoreactivity (green) appears in neurons (arrows). B. Pre-incubation of the antibody with ACE2 (extracellular) Blocking Peptide (BLP-AC012), suppressed staining. Cell nuclei are stained with DAPI (blue).
Indirect flow cytometry
  • Cell surface detection of ACE2 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line:
    Cell surface detection of ACE2 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-ACE2 (extracellular) Antibody (#AAC-012) (2.5μg) + goat-anti-rabbit-FITC.
References
  1. Wong, M. (2016). Handbook of Hormones, 263, e29D-4.
  2. Samavati, L. et al. (2020) Front. Cell. Infect. Microbiol., 5, 317.
  3. Lubbe, L. et al. (2020) Clin. Sci., 134, 2851.
Scientific background

The renin–angiotensin system is a central mechanism for blood pressure regulation through a diverse system of hormones and receptors. Angiotensin-converting enzyme (ACE) is an ectoenzyme that plays a role in the generation of Ang II by catalyzing the extracellular conversion of the decapeptide Ang I. Moreover, ACE degrades active bradykinin (BK), which play an important role in the control of blood pressure. ACE2 is an ACE homolog that was discovered in 2000 when two independent research groups cloned homologous ACE that could convert Ang I to Ang (1–9). Sequence analysis suggests that ACE and ACE2 exhibit 42% amino acid homology and ACE2 has evolved through gene duplication1.

ACE2 consist of N-terminal peptidase domain with seven potential N-linked glycosylation sites that bears the characteristic HEXXH motif of M2 metalloproteases. It is mainly expressed in the small intestine, testis, kidneys, heart, thyroid and adipose tissue and in the lungs. ACE 2 has a protective role in the cardiovascular system and in alveolar epithelial cells. In the lungs ACE2 has numerous physiological functions, most of which are protective against lung injury2.

ACE 2 was identified as the receptor for highly pathogenic SARS (severe acute respiratory syndrome) coronavirus including the SARS-CoV-2 that cause the coronavirus disease 2019 (COVID-19) global pandemic. SARS virus binding downregulates the cellular expression of ACE2, and the binding induces clathrin-dependent internalization of virus/receptor (SARS/ACE2) complex. Not only has ACE2 facilitated the invasion of SARS virus for rapid replication, but also ACE2 is depleted from the cell membrane and therefore the damaging effects of Ang II are enhanced, resulting in acute deterioration of lung tissues3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 02/01/2022

Anti-ACE2 (extracellular) Antibody (#AAC-012) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize ACE2 from rat, mouse and human samples.

For research purposes only, not for human use
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