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Anti-Adenosine A3 Receptor (extracellular)-FITC Antibody

ADORA3, A3AR, Adenosine Receptor A3

Cat #: AAR-035-F
Alternative Name ADORA3, A3AR, Adenosine Receptor A3
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
Immunogen
  • Peptide (C)GWNRKATLASSQN, corresponding to amino acid residues 149 - 161 of mouse ADORA3 (Accession Q61618). Extracellular, 2nd loop.
Accession (Uniprot) Number Q61618
Gene ID 11542
Homology Rat – 10 out of 13 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Specificity The antibody will not recognize adenosine A3 receptor from human samples.
Label Fluorescein isothiocyanate (FITC)
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #AAR-035), and direct flow cytometry (labeled antibody).
Applications: fc, lci
Direct flow cytometry
  • Cell surface detection of Adenosine A3 Receptor by direct flow cytometry in live intact mouse WEHI-231 B lymphocyte cell line:
    Cell surface detection of Adenosine A3 Receptor by direct flow cytometry in live intact mouse WEHI-231 B lymphocyte cell line:
    ___ Cells.
    ___ Cells + Rabbit IgG isotype control-FITC.
    ___ Cells + Anti-Adenosine A3 Receptor (extracellular)-FITC Antibody (#AAR-035-F), (2.5µg).
  • Cell surface detection of Adenosine A3 Receptor by direct flow cytometry in live intact mouse P815 mastocytoma cell line:
    Cell surface detection of Adenosine A3 Receptor by direct flow cytometry in live intact mouse P815 mastocytoma cell line:
    ___ Cells.
    ___ Cells + Rabbit IgG isotype control-FITC.
    ___ Cells + Anti-Adenosine A3 Receptor (extracellular)-FITC Antibody (#AAR-035-F), (5µg).
References
  1. Okusa, M.D. (2002) Am. J. Physiol. Renal Physiol282, F10.
  2. Fredholm, B.B. et al. (2001) Pharmacol. Rev53, 527.
  3. Nakata, H. (1989) J. Biol. Chem. 264, 16545.
  4. Baraldi, P.G. et al. (2006) Curr. Med. Chem. 13, 3467.
  5. Headrick, J.P. et al. (2003) Am. J. Physiol. Heart Circ. Physiol. 285, H1797.
  6. Madi, L. et al. (2004) Clin. Cancer Res. 10, 4472.
  7. Yehuda.S.P. et al. (2007) Pharmacol. Rev. 16, 1601.
Scientific background

Adenosine is an endogenous nucleoside generated locally in tissues under conditions of hypoxia, ischemia, or inflammation. It modulates a variety of physiological functions in many tissues including the brain and heart.1,2 Adenosine exerts its action via four specific adenosine receptors (also named P1 purinergic receptors): Adenosine A1 Receptor (A1AR), Adenosine A2A Receptor (A2AAR), Adenosine A2B Receptor (A2BAR), and Adenosine A3 Receptor (A3AR). The various adenosine receptors can be distinguished on the basis of their distinct molecular structures, distinct tissue distributions, and differential selectivity for adenosine analogs.1-4 All are integral membrane proteins and are members of the G protein-coupled receptor superfamily. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, a cytoplasmic carboxyl terminus, and a third intracellular loop important in binding G proteins.1-3

Expression of A3AR has been reported in the brain, kidney, liver, and heart. It plays a role in modulation of cerebral ischemia, asthma, and cell growth. In addition, recent studies have established a cardioprotective role for A3AR.5 Expression of A3AR was reported to be elevated in cancerous tissues as well as in auto-immune inflammatory diseases.6,7 A patent has been filed for the use of A3AR and A3AR agonist as a diagnostic marker for therapeutic treatments of cancer and other diseases.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 18/10/2021

Anti-Adenosine A3 Receptor (extracellular) Antibody (#AAR-035) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and flow cytometry applications. It has been designed to recognize Adenosine A3 from mouse and rat samples. The antibody will not recognize the receptor from human samples.

Anti-Adenosine A3 Receptor (extracellular)-FITC Antibody (#AAR-035-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. The antibody will not recognize the receptor from human samples.

For research purposes only, not for human use
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