Anti-Adenylate Cyclase 3 (AC3) (extracellular) Antibody

Adenylate cyclase type 3, Adenylate cyclase type III, AC-III, ATP pyrophosphate-lyase 3, Adenylate cyclase olfactive type, Adenylyl cyclase 3, ADCY3
    Cat #: AAR-043
    Alternative Name Adenylate cyclase type 3, Adenylate cyclase type III, AC-III, ATP pyrophosphate-lyase 3, Adenylate cyclase olfactive type, Adenylyl cyclase 3, ADCY3
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)KHVADEMLKDMKKDE, corresponding to amino acid residues 285-299 of rat ADCY3 (Accession P21932). 3rd extracellular loop.
    • Anti-Adenylate Cyclase 3 (AC3) (extracellular) Antibody
    Accession (Uniprot) Number P21932
    Gene ID 64508
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human - identical.
    RRID AB_2340889.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.85 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, lci, wb
    May also work in: ifc*, ip*
    Western blot
    • Anti-Adenylate Cyclase 3 (AC3) (extracellular) Antibody
      Western blot analysis of rat lung (lanes 1 and 4), brain (lanes 2 and 5) and hippocampus (lanes 3 and 6) lysates:
      1-3. Anti-Adenylate Cyclase 3 (AC3) (extracellular) Antibody (#AAR-043), (1:200).
      4-6. Anti-Adenylate Cyclase 3 (AC3) (extracellular) Antibody, preincubated with the negative control antigen.
    Immunohistochemistry
    • Anti-Adenylate Cyclase 3 (AC3) (extracellular) Antibody
      Expression of Adenylate cyclase type III in mouse hippocampal neurons
      Immunohistochemical staining of mouse hippocampal sections using Anti-Adenylate Cyclase 3 (AC3) (extracellular) Antibody (#AAR-043), (1:400), (red). A. AC3 staining of neurons. B. Staining with mouse anti-NeuN (green), as a neuronal marker on the same section. C. Merge of A and B demonstrates “primary cilia” - thin rod-like extensions from neurons in the pyramidal layer of the hippocampus.
    Live cell imaging / Immunocytochemistry
    • Anti-Adenylate Cyclase 3 (AC3) (extracellular) Antibody
      Expression of Adenylate cyclase type III in rat U-87 MG cells
      Cell surface detection of Adenylate cyclase type III in intact living rat U-87 MG cells. A. Extracellular staining of cells using Anti-Adenylate Cyclase 3 (AC3) (extracellular) Antibody (#AAR-043), (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Merge of A with live view of the cells.
    References
    1. Choi. E.J. et al. (1992) Biochemistry 31, 6492.
    2. Chern, Y. et al. (2000) Cell. Signal. 12, 195.
    3. Yang, B. et al. (1999) Biochem. Biophys. Res. Commun. 254, 548.
    4. Seed, A.M. et al. (2012) Islets 4, 343.
    5. Hong, S.H. et al. (2013) Oncotarget 4, 1791.
    6. Wong, S.T. et al. (2000) Neuron 27, 487.
    Scientific background

    Adenylyl cyclases (ACs) are a family of enzymes that synthesize cAMP, upon stimulation. cAMP is an important second messenger, which regulates carbohydrate, lipid, protein, and nucleic acid metabolism as well as synaptic transmission, ion channel function, and transcription in neurons1. There are nine closely related isoforms of ACs (AC1–9) in mammals. All AC isoforms share a primary structure consisting of two hydrophobic regions which comprise six transmembrane helices and three large cytoplasmic domains. The basic catalytic unit of a membrane-bound AC molecule consists of the C1a and C2 domains. The N-terminus domains of ACs are highly variable, and might play a regulatory role2.

    Adenylyl cyclase type 3 (AC3) is widely expressed in the olfactory tissue, human cell line HEK-293, bovine brain, spinal cord, adrenal medulla, adrenal cortex, heart atrium, aorta, lung and retina3.

    Several studies have suggested that AC3 may play an important role in the regulation of glucose metabolism and body weight. It was found that overexpression of AC3 mRNA is involved in pathophysiological metabolic processes, such as type 2 diabetes4. In addition, a recent study demonstrated the association of AC3 with gastric cancer as well as its tumorigenic potentials5.

    Furthermore, AC3-knockout mice showed peripheral and behavioral anosmia6.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Anti-Adenylate Cyclase 3 (AC3) (extracellular) Antibody (#AAR-043) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and live cell imaging applications. The antibody recognizes an extracellular epitope and thus is ideal for detecting ADCY3 in living cells. It has been designed to recognize ADCY3 from human, rat and mouse samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
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