Anti-AKAP12 Antibody

A-kinase anchoring protein 12, A-kinase anchor protein 250 kDa, AKAP250, Gravin, Myasthenia gravis autoantigen gravin, Src-suppressed C kinase substrate, SSeCKS

Cat #: APZ-033
Alternative Name A-kinase anchoring protein 12, A-kinase anchor protein 250 kDa, AKAP250, Gravin, Myasthenia gravis autoantigen gravin, Src-suppressed C kinase substrate, SSeCKS
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)GNLTKESPDTNGPK, corresponding to amino acid residues 1639-1652 of mouse AKAP12 (Accession Q9WTQ5). Intracellular, C-terminus.
Accession (Uniprot) Number Q9WTQ5
Gene ID 83397
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat – identical; human – 13/14 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
    Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
    1,2. Anti-AKAP12 Antibody (#APZ-033), (1:200).
    3,4. Anti-AKAP12 Antibody, preincubated with AKAP12 Blocking Peptide (#BLP-PZ033).
Immunohistochemistry
  • Expression of AKAP12 in rat cerebellum
    Expression of AKAP12 in rat cerebellum
    Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-AKAP12 Antibody (#APZ-033), (1:400), followed by goat anti-rabbit-AlexaFluor-488. AKAP12 immunoreactivity (green) is detected in Purkinje cells and their dendritic trees (arrows). Cell nuclei are stained with DAPI (blue).
References
  1. Carnegie, G.K. et al. (2009) IUBMB Life 61, 394.
  2. Gelman, I.H. (2010) Genes Cancer 1, 1147.
  3. Han, B. et al. (2015) Pflugers Arch. 467, 2401.
  4. Langeberg, L.K. and Scott, J.D. (2005) J. Cell Sci. 118, 3217.
  5. Uhlén, M. et al. (2015) Science 347, 1260419.
  6. Wild, A.R. and Dell’Acqua, M.L. (2018) Pharmacol. Ther. 185, 99.
  7. Wong, W. and Scott, J.D. (2004) Nat. Rev. Mol. Cell. Biol. 5, 959.
  8. Wu, X. et al. (2018) Biomed. Environ. Sci. 31, 927.
Scientific background

The A-kinase anchoring protein 12 (AKAP12) belongs to a family of scaffold proteins, which anchor signaling proteins such as PKA in a spatiotemporal manner4. AKAPs generally bind PKA at their N-terminus, while the sequence at their C-terminus determines their spatial location4. To date, more than 50 AKAPs have been identified and perceived to integrate signaling pathways by anchoring multi-protein complexes1.

AKAP12 integrates diverse signaling pathways such as PKA, PKC, and β2-adrenergic receptor7,8. Similar to other members of this protein-family, AKAP12 contains a PKA binding domain. In addition, AKAP12 also contains a docking site for PKC2,6 and a myristoyl acid that sequesters it to the plasma membrane7.

AKAP12 is widely expressed in a variety of tissues with the highest expression in male and female reproductive organs, skeletal muscle, kidneys, and urinary tract5.

One of the most characterized activities of AKAP12 is regulation of the cell cycle3. AKAP12 controls two fundamental cell processes such as the G1/S transition and cytokinesis. In that being said, studies in NIH-3T3 fibroblasts, showed that ectopic expression of AKAP12 reduced cyclin D1 levels and the phosphorylation of its downstream target Rb, and consequently attenuated cell-cycle progression3. Furthermore, in HeLa cells, knockdown of AKAP12 resulted in profound changes in cell morphology, which indicates the inability of cells to divide3.

Given that AKAP12 plays a pivotal role in cell cycle regulation, it is not surprising that in some cases it is involved in cancer malignancies. It is now clear that AKAP12 is a key driver of gastrointestinal cancers such as colorectal cancer and hepatocellular carcinomas8.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 25/07/2021

Anti-AKAP12 Antibody (#APZ-033) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Gravin from mouse, rat, and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Shipping and Ordering information