Anti-Angiotensin II Receptor Type-1 (extracellular)-ATTO-550 Antibody

AT1 Receptor, AT1R, AGTR1
    Cat #: AAR-011-AO
    Alternative Name AT1 Receptor, AT1R, AGTR1
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide NSSTEDGIKRIQDDC, corresponding to amino acid residues 4-18 of human AT1 (Accession P30556). Extracellular, N-terminus.
    • Anti-Angiotensin II Receptor Type-1 (extracellular)-ATTO-550 Antibody
    Accession (Uniprot) Number P30556
    Gene ID 185
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, rat, rabbit, dog - identical.
    RRID AB_2039721.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Label ATTO-550. Maximum absorption 554 nm; maximum fluorescence 576 nm. The fluorescence is excited most efficiently in the 540 - 565 nm range. This label is related to the dye Rhodamine 6G and can be used with filters used to detect Rhodamine.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #AAR-011), and immunohistochemistry (labeled antibody).
    Applications: ic, if, ih, lci
    May also work in: ifc*
    Immunohistochemistry
    • Anti-Angiotensin II Receptor Type-1 (extracellular)-ATTO-550 Antibody
      Expression of AGTR1 in rat kidney
      Immunohistochemical staining of rat kidney paraffin-embedded sections using Anti-Angiotensin II Receptor Type-1 (extracellular)-ATTO-550 Antibody (#AAR-011-AO), (1:50), (red). Staining is specific to the innermost layer of the cortex. Intense staining is present in proximal tubes (P) but not in collecting ducts (C) in the cortical labyrinths (CL). No staining is present both in thin portions of the Loop of Henle or in the collecting ducts in the medullar rays (MR). Hoechst 33342 (blue) is used to visualize the nuclei.
    • Anti-Angiotensin II Receptor Type-1 (extracellular)-ATTO-550 Antibody
      Expression of AGTR1 in rat cerebellum
      Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Angiotensin II Receptor Type-1 (extracellular)-ATTO-550 Antibody (#AAR-011-AO), (1:60). AT1R staining (orange) appears in the soma (upward arrows) and in the dendritic tree (downward arrows). Nuclei are labeled with DAPI (blue).
    Live cell imaging / Immunocytochemistry
    • Anti-Angiotensin II Receptor Type-1 (extracellular)-ATTO-550 Antibody
      Expression of Angiotensin II Receptor Type-1 in rat C6 glioma cells
      Cell surface detection of Angiotensin II Receptor Type-1 in live intact rat C6 glioma cells with Anti-Angiotensin II Receptor Type-1 (extracellular)-ATTO-550 Antibody (#AAR-011-AO), (1:50), (left panel). Right panel shows live view of the same field.
    References
    1. Sasaki, K. et al. (1991) Nature 351, 230.
    2. Murphy, T.J. et al. (1991) Nature 351, 233.
    3. De Gasparo, M. et al. (2000) Pharmacol. Rev. 52, 415.
    4. Hunyady, L. and Catt, K.J. (2006) Mol. Endocrinol. 20, 953.
    Scientific background

    Angiotensin II receptor type 1 (AT1, AGTR1) is one of the receptors that binds the octapeptide hormone Angiotensin II (Ang II).
     
    Ang II is the peptide hormone that generates most of the known effects of the renin-angiotensin system (RAS). Ang II is generated from the angiotensinogen protein by the actions of renin, angiotensin converting enzyme (ACE) and other peptidases. Ang II has a central role in cardiovascular homeostasis by regulating vasoconstriction, renal Na+ and water readsorption. In addition, Ang II induces cell growth and proliferation and has pro-inflammatory effects.
     
    Most of the physiological actions of Ang II are mediated by AT1 a member of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.

    The AT1 receptor is coupled to a Gq/11 protein that activates phospholipase C (PLC) and leads to production of inositol 1,4,5-trisphosphate (InsP3) and intracellular Ca2+ mobilization. In addition to the rapid actions mediated by InsP3 and Ca2+ signaling, AT1 receptor elicits other signaling responses including activation of the MAPK and Jak/STAT. Together, these signaling pathways mediate most of Ang II cellular responses that include, control of hormone secretion, regulation of cell growth and apoptosis, regulation of ion channels activation and cell migration.
     
    In accordance with the pleiotropic actions of Ang II, the AT1 receptor has a wide distribution with high levels detected in the adrenal gland, kidney, brain, heart, liver, etc.
     
    As is the case with many other peptide receptors, the AT1 receptor undergoes ligand-induced endocytosis, a process that contributes to receptor desensitization and sequestration and contributes to regulate receptor signaling.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title:

    Anti-Angiotensin II Receptor Type-1 (extracellular)-ATTO-550 Antibody
    Expression of AT1 receptor in rat diaphragm and carotid body.Immunohistochemical staining of rat diaphragm muscle and carotid artery sections using Anti-Angiotensin II Receptor Type-1 (extracellular)-ATTO-550 Antibody (#AAR-011-AO). A. AT1 staining (red) in diaphragm is detected in muscle fibers. AT1 staining in carotid artery is observed in smooth muscle. DAPI is used to stain nuclei (blue).Adapted from Kwon, O.S. et al. (2015) J. Appl. Physiol. 119, 1033. with permission of the American Physiological Society.

    Last update: 24/01/2020

    Anti-Angiotensin II Receptor Type-1 (extracellular) Antibody (#AAR-011) is a highly specific antibody directed against an extracellular epitope of human protein. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry and indirect flow cytometry applications, and will recognize AT1 receptor from rat, mouse and human samples.

    Anti-Angiotensin II Receptor Type-1 (extracellular)-ATTO-550 Antibody (#AAR-011-AO) is directly labeled with an ATTO-550 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-550 fluorescent label is related to the well known dye Rhodamine 6G and can be used with filters typically used to detect Rhodamine. Anti-Angiotensin II Receptor Type-1 (extracellular)-ATTO-550 Antibody has been tested in immunocytochemistry and immunohistochemistry applications and is especially suited for experiments requiring simultaneous labeling of different markers.

    For research purposes only, not for human use
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