Anti-Angiotensin II Receptor Type-2 (extracellular) Antibody

AT2 Receptor, AT2R, AGTR2, MRX88
Cat #: AAR-012
Alternative Name AT2 Receptor, AT2R, AGTR2, MRX88
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: m, r
      • Peptide DNLNATGTNESAFNC, corresponding to amino acid residues 21-35 of rat AT2 receptor (Accession P35351). Extracellular, N-terminal domain.
    Accession (Uniprot) Number P35351
    Gene ID 24182
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
    Specificity Will not recognize human AT2 receptor.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ih, lci, wb
    May also work in: ifc, ip
      • Western blot analysis of mouse MS1 endothelial cells:
        1. Anti-Angiotensin II Receptor Type-2 (extracellular) Antibody, (AAR-012), (1:400).
        2. Anti-Angiotensin II Receptor Type-2 (extracellular) Antibody, preincubated with the control peptide antigen.
        Western blot analysis of rat brain membranes:
        1. Anti-Angiotensin II Receptor Type-2 (extracellular) Antibody (AAR-012), (1:500).
        2. Anti-Angiotensin II Receptor Type-2 (extracellular) Antibody, preincubated with the control peptide antigen.
      • Expression of Angiotensin II receptor type-2 in rat brain
        Immunohistochemical staining of rat brain sections using Anti-Angiotensin Receptor Type-2 (extracellular) Antibody (#AAR-012). A. AT2 receptor expressing neurons (red) are scattered in the paraventricular nucleus of the hypothalamus (arrows), in the vicinity of the 3rd ventricle (3rd V). B. AT2 receptor expressing neurons in the supraoptic nucleus (arrows), adjacent to the optic nerve. Nissl is used as the counterstain (blue).
      • Expression of Angiotensin II receptor type-2 in rat GH3 pituitary cells
        Cell surface detection of Angiotensin II receptor type-2 in live intact rat GH3 pituitary cells using Anti-Angiotensin II Receptor Type-2 (extracellular) Antibody (#AAR-012), (1:100), followed by goat anti-rabbit-AlexaFluor-555 secondary antibody.
      • Angiotensin II receptor type 2 or AT2 is one of the receptors that bind the octapeptide hormone Angiotensin II (Ang II).
         
        Ang II is the peptide hormone that generates most of the known effects of the renin-angiotensin system (RAS). Ang II is generated from the angiotensinogen protein by the actions of renin, angiotensin converting enzyme (ACE) and other peptidases. Ang II has a central role in cardiovascular homeostasis by regulating vasoconstriction, renal Na+ and water readsorption. In addition, Ang II induces cell growth and proliferation and has pro-inflammatory effects.
         
        Most of the physiological actions of Ang II are mediated by AT1 a member of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. AT2 is also a GPCR, and is largely believed to have counter-regulatory roles to the ones exerted through the AT1 receptor. Hence, binding of Ang II to the AT2 receptor will result in growth arrest and apoptosis, vasodilatation and hypotension.
         
        Although the AT2 receptor is structurally a member of the GPCR superfamily, the signaling mechanisms elicited following AT2 receptor activation are not fully clarified. Recent evidence indicates that the AT2 receptor signals through the Giα2 and the Giα3 proteins and through the activation of phosphotyrosine phosphatases such as  SHP-1.
         
        AT2 receptor distribution is more restricted than that of the AT1 receptor. The highest expression levels of AT2 have been found in the fetus, which is followed by a marked decrease shortly after birth. Nevertheless, significant AT2 receptor expression can be detected in the heart, kidney, vascular endothelial cells and brain, among others.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 09/04/2019

    Anti-Angiotensin II Receptor Type-2 (extracellular) Antibody (#AAR-012) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry and immunohistochemistry applications. It has been designed to recognize AT2 receptor from rat and mouse samples. The antibody won’t recognize human AT2R.

    For research purposes only, not for human use
    Citations
      • Rat heart lysate (1:1000).
        Lino, C.A. et al. (2015) Mol. Cell. Endocrinol. 416, 1.
      • Guinea pig heart lysate (1:1000).
        Hardwick, J.C. et al. (2015) Am. J. Physiol. 309, R179.
      • Rat heart lysate (1:800).
        Tavares, F.M. et al. (2013) Cardiovasc. Drugs Ther. 27, 393.
      • Mouse lung lysate (1:1000).
        Rodrigues-Machado, M.G. et al. (2013) Br. J. Pharmacol. 170, 835.
      • Mouse tumour sections (1:200).
        Chauhan, V.P. et al. (2013) Nat. Commun. 4, 1.
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