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Anti-Anoctamin-6 (extracellular) Antibody

ANO6, Transmembrane protein 16F, TMEM16F, Small-conductance calcium-activated nonselective cation channel, SCAN channel
Cat #: ACL-016
Alternative Name ANO6, Transmembrane protein 16F, TMEM16F, Small-conductance calcium-activated nonselective cation channel, SCAN channel
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)KLPKNINGTDPIQK, corresponding to amino acid residues 487-500 of human ANO6 (Accession Q4KMQ2). 2nd extracellular loop.
Accession (Uniprot) Number Q4KMQ2
Gene ID 196527
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, rat – 12/14 amino acid residues identical.
RRID AB_2756564.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ifc, wb
May also work in: ic*, ih*, ip*, lci*
Western blot
  • Western blot analysis of mouse liver lysate (lanes 1 and 3) and rat liver membranes (lanes 2 and 4):
    Western blot analysis of mouse liver lysate (lanes 1 and 3) and rat liver membranes (lanes 2 and 4):
    1,2. Anti-Anoctamin-6 (extracellular) Antibody (#ACL-016), (1:200).
    3,4. Anti-Anoctamin-6 (extracellular) Antibody, preincubated with Anoctamin-6 (extracellular) Blocking Peptide (#BLP-CL016).
  • Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 3) and human MEG-01 megakaryoblastic leukemia cell line lysate (lanes 2 and 4):
    Western blot analysis of human THP-1 monocytic leukemia cell line lysate (lanes 1 and 3) and human MEG-01 megakaryoblastic leukemia cell line lysate (lanes 2 and 4):
    1,2. Anti-Anoctamin-6 (extracellular) Antibody (#ACL-016), (1:200).
    3,4. Anti-Anoctamin-6 (extracellular) Antibody, preincubated with Anoctamin-6 (extracellular) Blocking Peptide (#BLP-CL016).
Indirect flow cytometry
  • Cell surface detection of ANO6 in live human MEG-01 megakaryoblastic leukemia cell line:
    Cell surface detection of ANO6 in live human MEG-01 megakaryoblastic leukemia cell line:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-Anoctamin-6 (extracellular) Antibody (#ACL-016), 5µg + goat-anti-rabbit-FITC.
References
  1. Ousingsawat, J. et al. (2015) J. Biol. Chem. 290, 6270.
  2. Zhao, P. et al. (2014) PLoS One 9, e92749.
  3. Scudieri, P. et al. (2012) Exp. Physiol. 97, 177.
  4. Forschbach, V. et al. (2015) Cell Death Dis. 6, e1899.
  5. Mattheij, N.J. et al. (2016) FASEB J. 30, 727.
Scientific background

Anoctamin-6 (ANO6, TMEM16F) is a member of the Anoctamin chloride channel family. This family includes 10 proteins: ANO1-ANO10 that are encoded by the conserved TMEM16A-K genes. Anoctamin-6 acts as calcium (Ca2+)-activated chloride (Cl) channel. It generates an outwardly rectifying Cl current when activated by a large increase in local intracellular Ca2+ concentrations1. ANO6 structure includes eight transmembrane domains and cytosolic amino- and carboxyl termini. There is a high sequence identity in the putative transmembrane domains and more divergent in other regions2,3.

ANO6 has also been shown to operate as a Ca2+-activated phospholipid scramblase resulting in movement of phosphatidylserine from the inner to the outer leaflet of the plasma membrane bilayer. ANO6 is also involved in bone mineralization, cell volume regulation, cell proliferation and apoptosis1,2. ANO6 is expressed in cyst-forming epithelial cells together with ANO1.

A mutation in the ANO6 gene is linked to Scott Syndrome, a rare inherited bleeding disorder, characterized by altered Ca2+-dependent platelet signaling with defective phosphatidylserine exposure and microparticle formation. Deletion of ANO6 in mice shows reduced skeleton size, skeletal deformities, and mineralization defects4,5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Anti-Anoctamin-6 (extracellular) Antibody (#ACL-016) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, and live cell flow cytometry applications. It has been designed to recognize ANO6 from human, rat, and mouse samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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