Free shipping starts now, no minimum, no coupons required!

Anti-Apelin/APJ Receptor (extracellular) Antibody

APLNR, Angiotensin receptor-like 1, AGTRL1, G-protein coupled receptor APJ, GPCR34

Cat #: AAR-030
Alternative Name APLNR, Angiotensin receptor-like 1, AGTRL1, G-protein coupled receptor APJ, GPCR34
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide DGYNYYGADNQSEC, corresponding to amino acid residues 4-17 of rat apelin receptor (Accession Q9JHG3). Extracellular, N-terminus.
Accession (Uniprot) Number Q9JHG3
Gene ID 83518
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human – identical.
RRID AB_2756541.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.85 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ifc, ih, wb
May also work in: ic*, ip*, lci*
Western blot
  • Western blot analysis of mouse brain membrane (lanes 1 and 4), rat pituitary cell line (GH3) lysate (lanes 2 and 5) and rat heart membrane (lanes 3 and 6):
    Western blot analysis of mouse brain membrane (lanes 1 and 4), rat pituitary cell line (GH3) lysate (lanes 2 and 5) and rat heart membrane (lanes 3 and 6):
    1-3. Anti-Apelin/APJ Receptor (extracellular) Antibody (#AAR-030), (1:200).
    4-6. Anti-Apelin/APJ Receptor (extracellular) Antibody, preincubated with Apelin/APJ Receptor (extracellular) Blocking Peptide (#BLP-AR030).
Immunohistochemistry
  • Expression of Apelin receptor in mouse brain
    Expression of Apelin receptor in mouse brain
    Immunohistochemical staining of mouse cerebellum free floating frozen sections using Anti-Apelin/APJ Receptor (extracellular) Antibody (#AAR-030), (1:600). A. Apelin receptor staining (green) appears in neurons of the Purkinje cell (PC) (horizontal arrows in C) layer and in the dendritic tree (vertical arrow in C) in the molecular layer (MOL). B. Nuclei staining using DAPI as the counterstain (blue). C. Merged image of panels A and B.
Indirect flow cytometry
  • Cell surface detection of Apelin/APJ Receptor in live intact human THP-1 monocytic leukemia cells:
    Cell surface detection of Apelin/APJ Receptor in live intact human THP-1 monocytic leukemia cells:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-Apelin/APJ Receptor (extracellular) Antibody (#AAR-030), (2.5 µg) + goat-anti-rabbit-FITC.
References
  1. O'Carroll, A.M. et al. (2013) J. Endocrinol. 219, 13.
  2. Pitkin, S.L. et al. (2010) Pharmacol. Rev. 62, 331.
Scientific background

The apelin receptor (APJ) is a member of the G-protein coupled receptor gene family, encoded by the APLNR gene. The apelin receptor and its endogenous ligand, apelin, have been suggested to act as key mediators of physiological responses to multiple homeostatic actions, including cardiovascular control, water balance, hypothalamic–pituitary–adrenal (HPA) axis regulation and metabolic homeostasis.

Apelin receptor structure contains seven hydrophobic transmembrane domains, with several sites for phosphorylation by protein kinase A, and sites for palmitoylation and glycosylation. The N-terminal glycosylation is implicated in the receptor’s expression, stability, correct folding of the protein and ligand binding. The C-terminal palmitoylation has been reported to play a role in membrane association. These modifications can influence the internalization, dimerization and ligand binding of the receptor. Human apelin receptor shares 92% homology with mouse receptor and 90% homology with rat receptor.

Apelin expression can be detected in several brain regions including the human caudate nucleus, corpus callosum, hippocampus, substantia nigra, subthalamic nucleus, medulla and spinal cord. Apelin receptor is also expressed in the spleen, placenta and cardiovascular tissues and small amounts in the small intestine, lung, stomach, colonic mucosa and ovary.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 12/08/2021

Anti-Apelin/APJ Receptor (extracellular) Antibody (#AAR-030) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, live cell flow cytometry, and immunohistochemistry applications. It has been designed to recognize Apelin receptor from mouse, rat, and human samples.

For research purposes only, not for human use
Shipping and Ordering information