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Anti-Aquaporin 4 (AQP4) (300-314) Antibody

WCH4, Mercurial-insensitive water channel

Cat #: AQP-014
Alternative Name WCH4, Mercurial-insensitive water channel
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide (C)HVIDIDRGDEKKGKD, corresponding to amino acid residues 300-314 of rat AQP4 (Accession P47863). Intracellular, C-terminus.
Accession (Uniprot) Number P47863
Gene ID 25293
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - 14/15 amino acid residues identical; human - 13/15 amino acid residues identical.
RRID AB_11122614.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ih, wb
May also work in: ifc*, ip*
Western blot
  • Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
    Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates:
    1,2. Anti-Aquaporin 4 (AQP4) (300-314) Antibody (#AQP-014), (1:200).
    3,4. Anti-Aquaporin 4 (AQP4) (300-314) Antibody, preincubated with Aquaporin 4/AQP4 (300-314) Blocking Peptide (#BLP-QP014).
Immunohistochemistry
  • Expression of Aquaporin 4 in rat brain
    Expression of Aquaporin 4 in rat brain
    Immunohistochemical staining of rat brain using Anti-Aquaporin 4 (AQP4) (300-314) Antibody (#AQP-014), (1:200). A. Aquaporin 4 (green) is detected in blood vessels in layers 1-3 of rat neocortex. B. Astrocytic processes were stained using mouse anti glial fibrillary acidic protein (GFAP) (red) and DAPI (blue) was used to delineate the cortical layers. C. Merge of Aquaporin 4 and GFAP shows overlap along blood vessels (arrows) in agreement with the role of astrocytes and Aquaporin 4 in regulation of the blood-brain barrier.
Immunocytochemistry
  • Expression of Aquaporin 4 in rat glioma C6 cells
    Expression of Aquaporin 4 in rat glioma C6 cells
    Immunocytochemical staining of fixed and permeabilized rat glioma C6 cells. A. Cells were stained with Anti-Aquaporin 4 (AQP4) (300-314) Antibody (#AQP-014), (1:200), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Cell nuclei were visualized using Hoechst 33342 (blue). C. Merge of the two images.
References
  1. King, L.S. et al. (2004) Nat. Rev. Mol. Cell Biol. 5, 687.
  2. Manley, G.T. et al. (2004) Neuroscience. 129, 983.
  3. Lennon, V.A. et al. (2005) J. Exp. Med. 202, 473.
Scientific background

Aquaporin 4 (AQP-4) belongs to a family of membrane proteins that allow passage of water and certain solutes through biological membranes. The family is composed of 13 members (AQP-0 to AQP-12).

The aquaporins can be divided into two functional groups based on their permeability characteristics: the aquaporins that are only permeated by water and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. AQP-4 together with AQP-1, AQP-2 and AQP-5 belong to the first group1. Little is known about the function of the two newest members, AQP-11 and AQP-12.

The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit, contains four pores1.

AQP-4 is the major membrane water channel in the central nervous system. The channel is expressed in astrocyte foot processes in direct contact with capillary vessels in the brain suggesting a role in water transport under normal and pathological conditions. Indeed, transgenic mice lacking AQP-4 have reduced brain swelling and improved neurological outcome following water intoxication and focal cerebral ischemia. In contrast, brain swelling and clinical outcome are worse in AQP-4-null mice in models of vasogenic (fluid leak) edema caused by freeze-injury and brain tumor, probably due to impaired AQP-4-dependent brain water clearance2.

In addition, it has been recently shown that neuromyelitis optica (NMO), an inflammatory demyelinating disease that selectively affects optic nerves and spinal cord, is caused by the development of an autoantibody directed against AQP-43.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Anti-Aquaporin 4 (AQP4) (300-314) Antibody (#AQP-014) is a highly specific antibody directed against an epitope of rat AQP4. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize the Aquaporin 4 channel from rat, mouse, and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Immunohistochemistry citations
  1. Mouse spinal cord sections.
    Dai, J. et al. (2017) Mol. Med. Rep. 16, 1739.
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