Overview
- Peptide ENIQSVLQKTWVREFLAE, corresponding to amino acid residues 7-24 of rat AQP7 (Accession P56403). Intracellular, N-terminus.
Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) kidney membranes:1,2. Anti-Aquaporin 7 Antibody, (#AQP-007), (1:500).
3,4. Anti-Aquaporin 7 Antibody, preincubated with Aquaporin 7 Blocking Peptide (#BLP-QP007).
Expression of AQP7 in rat kidneyImmunohistochemical staining of paraffin-embedded rat kidney sections using Anti-Aquaporin 7 Antibody (#AQP-007), (1:100). A. In the minor calyx, AQP7 staining (deep red) is specific for epithelial cells of the uroepithelium B. In the cortical labyrinth, AQP7 staining is specific for renal tubules while the stronger staining is evident in the brush border (blue arrows). Hematoxilin is used as the counterstain.
Expression of AQP7 in rat adipocytesImmunocytochemical staining of 3T3-L1 adipocytes using Anti-Aquaporin 7 Antibody (#AQP-007), (1:800), followed by goat anti-rabbit-AlexaFluor-555 secondary antibody (red) A, C. Anti-Aquaporin 7 Antibody preincubated with the control peptide antigen followed by goat-anti-rabbit-AlexaFluor-555 secondary antibody. B, D. Visible light images of the adipocytes shown in (A) and (C) respectively.
- Kuriyama, H. et al. (1997) Biochem. Biophys. Res. Commun. 241, 53.
- King, L.S. et al. (2004) Nat. Rev. Mol. Cell Biol. 5, 687.
- Fruhbeck, G. et al. (2006) Trends Pharmacol. Sci. 27, 345.
Aquaporin 7 (AQP-7) belongs to a family of membrane proteins that allow passage of water and certain other solutes through biological membranes. The family is composed of 13 members (AQP-0 to AQP-12). Little is known about the function of the two newest members, AQP-11 and AQP-12.
The aquaporins can be divided into two functional groups based on their permeability characteristics: the aquaporins that are only permeated by water and the aquaglyceroporins that are permeated by water and other small solutes such as glycerol. This last group includes AQP-7, AQP-3, AQP-9 and AQP-10.
The proteins present a conserved structure of six transmembrane domains with intracellular N- and C-termini. The functional channel is a tetramer but each subunit has a separate pore and therefore the functional channel unit contains four pores.
AQP-7 is expressed in ovary, testis, kidney and adipose tissue.
The function of AQP-7 in adipose tissue attracted much interest as mice deficient in AQP-7 developed adult-onset obesity and Type 2 Diabetes. AQP-7 modulates adipocyte glycerol permeability thereby controlling triglyceride accumulation and fat cell size.
Anti-Aquaporin 7 Antibody (#AQP-007) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize AQP-7 from mouse and rat samples, and may recognize the channel from human samples.
Applications
Citations
Powered by Bioz- Rat kidney lysate.
Chung, S. et al. (2019) Front. Physiol. 10, 271.
- Rat kidney sections.
Chung, S. et al. (2019) Front. Physiol. 10, 271.
- Best, L. et al. (2009) Cell. Physiol. Biochem. 23, 255.
- Delporte, C. et al. (2009) J. Cell. Physiol. 221, 424.
