Guinea pig Anti-ASIC1 Antibody

Acid-sensing ion channel 1, Amiloride-sensitive brain sodium channel, BNaC2, ACCN2
    Cat #: AGP-053
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Guinea pig
    Reactivity: h, m, r
    Immunogen
    Peptide CQKEAKRSSADKGVALSLDD, corresponding to amino acid residues 469-488 of rat ASIC1 (Accession P55926). Intracellular, C-terminus.
    Accession (Uniprot) Number P55926
    Gene ID 79123
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human - identical; mouse - 19/20 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Specificity This antibody will recognize all known ASIC1 isoforms.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ih, wb
    May also work in: ifc, ip
    Western blot
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:
    1,2. Guinea pig Anti-ASIC1 Antibody (#AGP-053), (1:600).
    3,4. Guinea pig Anti-ASIC1 Antibody, preincubated with the control peptide antigen.
    Western blot analysis of human SH-SY5Y neuroblastoma cell line lysate:
    1. Guinea pig Anti-ASIC1 Antibody (#AGP-053), (1:600).
    2. Guinea pig Anti-ASIC1 Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    Immuno-colocalization of ASIC1 and parvalbumin in rat hippocampus
    Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Guinea pig Anti-ASIC1 Antibody (#AGP-053), (1:300). A. ASIC1 (green) is detected in the hilus of the dentate gyrus (arrows). B. The same section was stained with anti-parvalbumin (red). C. Merge of the two images shows partial colocalization (orange, see arrows). Cell nuclei in the same section were stained with DAPI (Blue).
    Immunocytochemistry
    Expression of ASIC1 in rat C6 glioma cells
    Immunocytochemical staining of fixed and permeabilized C6 glioma cells. A. Cells were stained using Guinea pig Anti-ASIC1 Antibody (#AGP-053), (1:200) followed by anti-guinea pig-AlexaFluor-594 secondary antibody (red). B. Cell nuclei were visualized using Hoechst 33342 (blue). C. Merge of the two images.
    References
    1. Kellenberger, S. et al. (2002) Physiol. Rev. 82, 735.
    2. Wemmie, J.A. et al. (2002) Neuron 34, 463.
    3. Wemmie, J.A. et al. (2003) J. Neurosci. 23, 5496.
    Scientific background

    ASIC1 is a member of a family of Na+ channels that are activated by external protons. The family includes another three members ASIC2ASIC3 and ASIC4.

    The ASICs are in fact part of a larger superfamily termed epithelial Na+ channels (EnaC) and share with it the same basic characteristics: two transmembrane spanning domains, a large extracellular domain and short intracellular N and C termini.  

    There are two recognized splice variants of the ASIC1 gene that differ on their N-termini, ASIC1a and ASIC1b that have different tissue distributions and functions.  

    The ASIC1 responds to a decrease in extracellular pH with an inward cation current that is quickly inactivated despite the continuous presence of protons in the medium.  

    Lately, ASIC1 has been implicated in processes such as learning and memory in the central nervous system.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title:

    Guinea pig Anti-ASIC1 Antibody
    Immuno-colocalization of ASIC1 and ASIC3 in rat DRG
    Immunohistochemical staining of perfusion-fixed frozen rat dorsal root ganglia (DRG) sections using Guinea pig Anti-ASIC1 Antibody (#AGP-053), (1:400) and rabbit Anti-ASIC3 Antibody (#ASC-018), (1:400). A. ASIC3 staining. B. ASIC1 staining in same section (green). C. Merge of the two images shows some co-localization (arrow). Cell nuclei were stained with DAPI (blue).

    Last update: 05/12/2018

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat ASIC1. Guinea pig Anti-ASIC1 Antibody (#AGP-053) raised in guinea pig can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize ASIC1 from mouse, rat, and human samples. The antigen used to immunize guinea pigs is the same as Anti-ASIC1 Antibody (#ASC-014) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as immuno-colocalization studies, immunoprecipitation, etc.

    For research purposes only, not for human use