This product is freeze dried. All water molecules have been removed.
This antibody is shipped with its antigen FREE of charge!
This lyophilized product is shipped at room temperature. Please see its certificate of analysis for further storage instructions.
3,4. Guinea pig Anti-ASIC1 Antibody, preincubated with the control peptide antigen.
2. Guinea pig Anti-ASIC1 Antibody, preincubated with the control peptide antigen.
ASIC1 is a member of a family of Na+ channels that are activated by external protons. The family includes four additional members: ASIC2, ASIC3, ASIC4 and ASIC5. The ASICs are in fact part of a larger superfamily named degenerin/epithelial Na+ channels (DEG/ENaC) and share with it the same basic characteristics: two transmembrane spanning domains, a large extracellular domain and short intracellular N- and C-termini.
There are two recognized splice variants of the ASIC1 gene that differ on their N-termini, ASIC1a and ASIC1b that have different tissue distributions and functions.
ASIC1 responds to a decrease in extracellular pH with an inward cation current that is quickly inactivated despite the continuous presence of protons in the medium.
Recently, ASIC1 has been implicated in cognitive processes such as learning and memory.
Species reactivity key:
Immuno-colocalization of ASIC1 and ASIC3 in rat DRGImmunohistochemical staining of perfusion-fixed frozen rat dorsal root ganglia (DRG) sections using Guinea pig Anti-ASIC1 Antibody (#AGP-053), (1:400) and rabbit Anti-ASIC3 Antibody (#ASC-018), (1:400). A. ASIC3 staining. B. ASIC1 staining in same section (green). C. Merge of the two images shows some co-localization (arrow). Cell nuclei were stained with DAPI (blue).
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat ASIC1. Guinea pig Anti-ASIC1 Antibody (#AGP-053) raised in guinea pig can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize ASIC1 from mouse, rat, and human samples. The antigen used to immunize guinea pigs is the same as Anti-ASIC1 Antibody (#ASC-014) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as immuno-colocalization studies, immunoprecipitation, etc.
- Mouse DRG sections (1:250).
Reed-Geaghan, E.G. et al. (2016) J. Neurosci. 36, 4362.
- Blockers/Antagonists: peptides/peptide toxinsBlockers/Antagonists: small moleculesActivators/Agonists: peptides/peptide toxinsActivators/Agonists: small moleculesModulators: peptides/peptide toxins