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Anti-ASIC2a-ATTO Fluor-594 Antibody

ACCN1, Acid-sensing ion channel 2, Brain sodium channel 1, BNaC1, BNC1, Amiloride-sensitive cation channel neuronal 1, Mammalian degenerin homolog, MDEG
Cat #: ASC-012-AR
Alternative Name ACCN1, Acid-sensing ion channel 2, Brain sodium channel 1, BNaC1, BNC1, Amiloride-sensitive cation channel neuronal 1, Mammalian degenerin homolog, MDEG
  • KO Validated
  • Lyophilized Powder yes
    Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide DLKESPSEGSLQPSSIQC, corresponding to amino acid residues 2-18 of human ASIC2a (Accession Q16515). Intracellular, N-terminus.
    Accession (Uniprot) Number Q16515
    Gene ID 40
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse- identical.
    RRID AB_2341067.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Label ATTO-594. Maximum absorption 601 nm; maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label is related to the Rhodamine dyes and can be used with filters used to detect Texas Red and Alexa-594.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #ASC-012), and immunohistochemistry (labeled antibody).
    Applications: if, ih
    May also work in: ic*
    Immunohistochemistry
    • Expression of ASIC2a in rat cerebellum and spinal cord
      Expression of ASIC2a in rat cerebellum and spinal cord
      Immunohistochemical staining of perfusion-fixed frozen rat brain and spinal cord sections using Anti-ASIC2a-ATTO Fluor-594 Antibody (#ASC-012-AR), (1:60). A. Staining in rat cerebellum sections reveals ASIC2a expression (red) in the molecular layer (MOL) of the cerebellar cortex. B. Staining in rat spinal cord sections shows labeling (red) of neuronal soma (horizontal arrow) in the ventral horn of the spinal cord. DAPI (blue) is used as counterstain in A and B.
    References
    1. Kellenberger, S. et al. (2002) Physiol. Rev. 82, 735.
    2. Krishtal, O. et al. (2003) Trends Neurosci. 26, 477.
    3. Price, M.P. et al. (2000) Nature 407, 1007.
    Scientific background

    ASIC2a is a member of a family of Na+ channels that are activated by external protons. The family includes four additional members: ASIC1, ASIC3ASIC4 and ASIC5. The ASICs are in fact part of a larger superfamily named degenerin/epithelial Na+ channels (DEG/ENaC) and share with it the same basic characteristics: two transmembrane spanning domains, a large extracellular domain and short intracellular N- and C-termini.

    There are two recognized splice variants of the ASIC2 gene that differ on their N-termini, ASIC2a and ASIC2b that have different tissue distributions and functions. ASIC2a is highly expressed in the central nervous system (CNS) and is less expressed in the peripheral nervous system (PNS) while ASIC2b is prominently expressed in the latter.

    The functional channel is composed of 4 subunits that can be assembled as homo- or heterotetramers with the other ASIC subunits. The ASIC2b splice variant does not appear to be functional when expressed alone but it can modify the properties of ASIC2a and ASIC3 when co-expressed.

    The ASIC2 protein has been proposed to be involved in mechanosensation and sensory transduction.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 23/08/2020

    Anti-ASIC2a Antibody (#ASC-012) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize ASIC2a from human, rat, and mouse samples. The antibody is specific for ASIC2a and will not recognize the ASIC2b variant.

    Anti-ASIC2a-ATTO Fluor-594 Antibody (#ASC-012-AR) is directly labeled with ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-ASIC2a-ATTO Fluor-594 Antibody is specially suited to experiments requiring simultaneous labeling of different markers.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    KO validation citations
    1. Western blot analysis of human primary astrocyte lysate using #ASC-012. Tested in siRNA-treated cells.
      Vila-Carriles, W.H. et al. (2007) J. Biol. Chem. 282, 34381.
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