Free shipping starts now, no minimum, no coupons required!

Anti-ATP1B3 (extracellular) Antibody

Sodium/Potassium-Transporting ATPase Subunit Beta-3, CD298, Beta 3 Na+/K+ ATPase
Cat #: ANP-013
Alternative Name Sodium/Potassium-Transporting ATPase Subunit Beta-3, CD298, Beta 3 Na+/K+ ATPase
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)NDEVPKYRDQIPSPG, corresponding to amino acid residues 66 - 80 of human Beta 3 Na+/K+ ATPase (Accession P54709). Extracellular, C-terminus.
Accession (Uniprot) Number P54709
Gene ID 483
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, rat – identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ifc, wb
May also work in: ic*, ih*, ip*, lci*
Western blot
  • Western blot analysis of human Jurkat T-cell leukemia cell line lysates (lanes 1 and 3) and human THP-1 monocytic leukemia cell line lysates (lanes 2 and 4):
    Western blot analysis of human Jurkat T-cell leukemia cell line lysates (lanes 1 and 3) and human THP-1 monocytic leukemia cell line lysates (lanes 2 and 4):
    1, 2. Anti-ATP1B3 (extracellular) Antibody (#ANP-013), (1:200).
    3, 4. Anti-ATP1B3 (extracellular) Antibody, preincubated with ATP1B3 (extracellular) Blocking Peptide (#BLP-NT013) (#BLP-NT013).
  • Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):
    Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4):
    1, 2. Anti-ATP1B3 (extracellular) Antibody (#ANP-013), (1:200).
    3, 4. Anti-ATP1B3 (extracellular) Antibody, preincubated with ATP1B3 (extracellular) Blocking Peptide (#BLP-NT013) (#BLP-NT013).
Indirect flow cytometry
  • Cell surface detection of ATP1B3 in live intact human THP-1 monocytic leukemia cells:
    Cell surface detection of ATP1B3 in live intact human THP-1 monocytic leukemia cells:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-ATP1B3 (extracellular) Antibody (#ANP-013), (2.5 µg) + goat-anti-rabbit-FITC.
References
  1. Malik, N. et al. (1998) Mamm. Genome 9, 136.
  2. Lingrel, J.B. (1992) J. Bioenerg. Biomembr. 24, 263.
  3. McDonough, A.A. et al. (1990) FASEB J. 4, 1598.
  4. Arystarkhova, E. and Sweadner, K.J. et al. (1997) J. Biol. Chem. 272, 22405.
Scientific background

Na+/K+ ATPase enzyme is composed of two subunits, a large catalytic subunit (α) and a smaller glycoprotein subunit (β). The subunits assemble into a heterodimer localized to the plasma membrane1. There are three α subunits and three β subunits expressed in a tissue- and developmental-specific manner2.

The Na+/K+ ATPase β subunit is composed of 302-304 amino acids, depending of the species or tissue location. The polypeptide is a single pas transmembrane protein. The protein structure is primarily helical from the amino terminus until just before the first glycosylation site3.

Beta 3 Na+/K+ ATPase (ATP1B3) is highly expressed in rat lung and testis. It is also present in liver and skeletal muscle4.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Check out more of our new products.

Last update: 23/08/2020

Anti-ATP1B3 (extracellular) Antibody (#ANP-013) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize ATP1B3 from rat, mouse, and human samples.

For research purposes only, not for human use
Shipping and Ordering information