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- Peptide (C)GRVRTYQFDSFLESTR, corresponding to amino acid residues 97-112 of mouse BAI1 (Accession Q3UHD1). Extracellular, N-terminus.
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:1,2. Anti-BAI1 (extracellular) Antibody (#ABR-021), (1:200).
3,4. Anti-BAI1 (extracellular) Antibody, preincubated with the negative control antigen.Western blot analysis of human HL-60 promyelocytic leukemia cell lysates:1. Anti-BAI1 (extracellular) Antibody (#ABR-021), (1:200).
2. Anti-BAI1 (extracellular) Antibody, preincubated with the negative control antigen.
- Expression of BAI1 in mouse olfactory bulbImmunohistochemical staining of mouse perfusion-fixed olfactory bulb frozen sections using Anti-BAI1 (extracellular) Antibody (#ABR-021), (1:200). A. BAI1 (green) is expressed in astrocyte-like cells (arrows). B. Double-staining of BAI1 (green) and glial fibrillary acidic protein (red) reveals expression of BAI1 in a subset of astrocytes. Nuclear staining of cells using the DNA dye DAPI (blue).
- Cell surface detection of BAI1 in live intact human HL-60 promyelocytic leukemia cell line:___ Unstained cells + goat-anti-rabbit-AlexaFluor-488 secondary antibody.
___ Cells + Anti-BAI1 (extracellular) Antibody (#ABR-021), (1:20) + goat-anti-rabbit-AlexaFluor-488 secondary antibody.The negative control antigen is not suitable for this application.
- 1. Park, D. et al. (2007) Nature 450, 430.
- 2. Cork, S.M. et al. (2011) J. Mol. Med. 89, 743.
- 3. de Fraipont, F. et al. (2001) Trends Mol. Med. 7, 401.
- 4. Oda, K. et al. (1999) Cytogenet. Cell. Genet. 84, 75.
- 5. Cork, S.M. et al. (2012) Oncogene 31, 5144.
- 6. Shiratsuchi, T. et al. (1997) Cytogenet. Cell. Genet. 79, 103.
- 7. Hatanaka, H. et al. (2000) Int. J. Mol. Med. 5, 181.
- 8. Fukushima, Y. et al. (1998) Int. J. Oncol. 13, 967.
The three members of the brain angiogenesis inhibitor (BaI1-3) are receptors belonging to the adhesion subfamily of G-protein coupled receptor superfamily. Like all members of GPCRs, all three BaIs have seven transmembrane domains, an intracellular C-terminal tail and extracellular N-terminus. Like other adhesion members, the N-terminus is quite large1,2. Many domains are localized to the N-terminus; various glycosylations sites are present, there is a GPCR proteolysis site, a putative hormone binding domain and thrombospondin type 1 repeats which regulate the anti-angiogenic activity of thrombospondin-12,3. The C-terminal tail interacts with PDZ-domain proteins. Unique to BaI1 is a proline-rich domain required for interacting with Src homology domains and WW domain proteins2,4.
Like most adhesion GPCRs, BaI also undergo proteolysis at the N-terminus at a highly rich cystein domain2. Following autocleavage, the N-terminal fragment remains associated to the receptor. In BaI1, proteolysis yields a partly secreted 120 kDa. fragment (vasculostatin-120) or a 40 kDa. fragment both having antiangiogenic effects2,5.
At the mRNA level, all BaIs are expressed in fetal and adult human brain2,6. BaI2 is detected in the human heart and skeletal muscle. BaI3 is expressed in the human heart, testis and small intestine. In mouse, both BaI2 and BaI3 are restricted to the brain2.
These receptors are implicated in various diseases and disorders such as primary glioma, pulmonary adenocarcinomas, gastric and colorectal cancers2,6,7.
Species reactivity key:
Anti-BAI1 (extracellular) Antibody (#ABR-021) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry, and indirect live cell flow cytometry applications. It has been designed to recognize BAI1 from rat, mouse, and human samples.