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Anti-Bestrophin-2 (extracellular) Antibody

BEST2, Vitelliform macular dystrophy 2-like protein 1, VMD2L1

Cat #: ABC-002
Alternative Name BEST2, Vitelliform macular dystrophy 2-like protein 1, VMD2L1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)DPAQGYKDHTLD, corresponding to amino acid residues 259-270 of mouse Bestrophin-2 (Accession Q8BGM5). 3rd extracellular loop.
Accession (Uniprot) Number Q8BGM5
Gene ID 212989
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat - identical; human - 11/12 amino acid residues identical.
RRID AB_10556895.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of rat colon membranes:
    Western blot analysis of rat colon membranes:
    1. Anti-Bestrophin-2 (extracellular) Antibody (#ABC-002), (1:200).
    2. Anti-Bestrophin-2 (extracellular) Antibody, preincubated with Bestrophin-2 (extracellular) Blocking Peptide (#BLP-BC002).
  • Western blot analysis of human colon cancer cell lines Colo-205 (lanes 1 and 4), human T-84 colonic adenocarcinoma cell line (lanes 2 and 5) and human colon cancer HT-29 cell line (lanes 3 and 6):
    Western blot analysis of human colon cancer cell lines Colo-205 (lanes 1 and 4), human T-84 colonic adenocarcinoma cell line (lanes 2 and 5) and human colon cancer HT-29 cell line (lanes 3 and 6):
    1-3. Anti-Bestrophin-2 (extracellular) Antibody (#ABC-002), (1:200).
    4-6. Anti-Bestrophin-2 (extracellular) Antibody, preincubated with Bestrophin-2 (extracellular) Blocking Peptide (#BLP-BC002).
Immunohistochemistry
  • Expression of Bestrophin-2 in rat colon
    Expression of Bestrophin-2 in rat colon
    Immunohistochemical staining of rat colon paraffin embedded sections using Anti-Bestrophin-2 (extracellular) Antibody (#ABC-002), (1:100), (brown). BEST2 is expressed in the columnar absorptive cells of the colonic epithelium (arrows). Hematoxilin is used as the counterstain.
References
  1. Men, G. et al. (2004) Am. J. Ophtalmol. 137, 963.
  2. Petrukhin, K. et al. (1998) Nat. Genet. 19, 241.
  3. Hartzell, C.H. et al. (2008) Physiol. Rev. 88, 639.
  4. Kramer, F. et al. (2004) Cytogen. Genome Res. 105, 107.
  5. Stohr, H. et al. (2002) Eur. J. Hum. Genet. 10, 281.
  6. Park, H. et al. (2009) J. Neurosci. 29, 13063.
  7. Pifferi, S. et al. (2006) Proc. Natl. Acad. Sci. U.S.A. 103, 12929.
  8. Tsunenari, T. et al. (2003) J. Biol. Chem. 278, 41114.
  9. Milenkovic, V.M. et al. (2007) J. Biol. Chem. 282, 1313.
  10. Xia, Q. et al. (2008) J. Gen. Physiol. 132, 681.
  11. Chien, L.T. et al. (2006) J. Gen. Physiol. 128, 247.
  12. Tsunenari, T. et al. (2006) J. Gen. Physiol. 127, 749.
  13. Qu, Z. et al. (2003) J. Biol. Chem. 278, 49563.
Scientific background

Mammalian Cl- channels can be broadly classified into four different families: voltage-dependent Cl- channels (CLCs), the cystic fibrosis transmembrane conductance regulator (CFTR), ligand-gated Cl- channels (γ-aminobutyric acid (GABA)) and glycine channels) and Ca2+-activated Cl- channels (Bestrophin and Anoctamin channels).

Bestrophins were first found by genetic linkage of human-Bestrophin-1 (hBest1) to a juvenile form of macular degeneration called Best vitelliform macular dystrophy1,2. To date Bestrophin 1-4 have been identified, although Bestrophin-3 and Bestrophin-4 have been observed only at the RNA level3. In addition, splice variants of some of these Ca2+-activated Cl- channels (CaCCs) have also been detected2,4,5. CaCCs are known to be involved in the regulation of olfaction, taste, phototransduction, and excitability in the nervous system. However, the molecular identity and functional role of CaCC in the brain have not been well established6. Bestrophin-2 is prominently expressed in colon and testes. Recently, Bestrophin-2 has also been found to be expressed in olfactory sensory neurons (OSNs)7.

Two different topologies for Bestrophin-1 have been proposed. The first structure proposes that six hydrophobic domains span the membrane8, while the second suggests that there are only four membrane-spanning domains9. The same structural controversy applies to Bestrophin-2. Bestrophin-1 and Bestrophin-2 (as well as Bestrophin-4 by heterologous expression) are activated by intracellular Ca2+ 10-12. Ca2+-dependent activation of Bestrophin-2 was also demonstrated in Xenopus13.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 08/01/2023

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope located at the 3rd extracellular loop of mouse Bestrophin-2. Anti-Bestrophin-2 (extracellular) Antibody (#ABC-002) can be used in western blot and immunohistochemical applications, and has been designed to recognize bestrophin-2 channel from mouse, rat and human samples.

For research purposes only, not for human use

Applications

Citations

Citations
Western blot citations
  1. Mouse colon epithelia lysate.
    Rottgen, T.S. et al. (2018) Am. J. Physiol. 315, C10.

Specifications

Scientific Background

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