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Anti-Beta 2 Na+/K+ ATPase (extracellular) Antibody

ATP1B2, Sodium/potassium-transporting ATPase subunit beta-2, Adhesion molecule on glia, AMOG

Cat #: ANP-012
Alternative Name ATP1B2, Sodium/potassium-transporting ATPase subunit beta-2, Adhesion molecule on glia, AMOG
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)DTESWDQHVQKLNK, corresponding to amino acid residues 99-112 of rat ATP1B2 (Accession P13638). Extracellular, C-terminus.
Accession (Uniprot) Number P13638
Gene ID 24214
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Human – identical; mouse 13/14 amino acid residues identical.
RRID AB_2756682.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*, lci*
Western blot
  • Western blot analysis of rat brain lysate:
    Western blot analysis of rat brain lysate:
    1. Anti-Beta 2 Na+/K+ ATPase (extracellular) Antibody (#ANP-012), (1:400).
    2. Anti-Beta 2 Na+/K+ ATPase (extracellular) Antibody, preincubated with β 2 Na+/K+ ATPase (extracellular) Blocking Peptide (#BLP-NP012).
  • Western blot analysis of mouse brain lysate:
    Western blot analysis of mouse brain lysate:
    1. Anti-Beta 2 Na+/K+ ATPase (extracellular) Antibody (#ANP-012), (1:200).
    2. Anti-Beta 2 Na+/K+ ATPase (extracellular) Antibody, preincubated with β 2 Na+/K+ ATPase (extracellular) Blocking Peptide (#BLP-NP012).
Immunohistochemistry
  • Expression of Na+/K+ ATPase β2 (ATP1B2) in mouse hippocampus
    Expression of Na+/K+ ATPase β2 (ATP1B2) in mouse hippocampus
    Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Beta 2 Na+/K+ ATPase (extracellular) Antibody (#ANP-012), (1:300), followed by goat anti-rabbit-AlexaFluor-488. ATP1B2 staining (green) in the hippocampal CA1 region, is detected in glial cell profiles (horizontal arrows) in the stratum radiatum (SR) and pyramidal (Pyr) layer. Cell nuclei are stained with DAPI (blue).
References
  1. Morth, J.P. et al. (2011) Nat. Rev. Mol. Cell Biol. 12, 60.
  2. Zhang, L.N. et al. (2012) Fundam. Clin. Pharmacol. 27, 96.
Scientific background

P-type ATPases are a large family of molecular pumps that exploit a phosphorylated enzyme intermediate in a two-step mechanism of ATP hydrolysis, cycling through states which are associated with ion transport or ion counter-transport. The Na+/K+-ATPase, a member of this family, is almost exclusively found in animals, although close homologues have been reported in certain archaea, algae and oomycetes.

The Na+/K+-ATPase is comprised of a nucleotide-binding (N) and phosphorylation (P) domain, a transmembrane core (M1–M6) and a large carboxy-terminal M7–M10 segment. Na+/K+-ATPase undergoes large conformational changes as part of its functional cycle giving rise to two distinct enzymatic states: E1, which is a high-affinity state for the primary transported ion- Na+ and E2, which is the low-affinity state for the Na+ ion. The two states arise from the autocatalysed formation and breakdown of a phosphoenzyme intermediate, coupled to the binding, occlusion, translocation and release of ions. The phosphorylation site is the Asp residue of a conserved DKTGT motif. The core of the membrane transport domain encompasses transmembrane helices M1–M6, which hold the main ion-binding sites and are necessary for cytoplasmic and extracellular ion transport. A terminal R domain extension, which serves as a regulatory unit, can be found in the C-terminal of the protein. This unit is auto-inhibitory and is predicted to restrict transmembrane helix movements and/or access of ions to the membrane transport core1.  

Na+/K+-ATPase has been implicated in the pathogenesis of Alzheimer’s Disease. A deficiency in several Na+/K+-ATPase isoform genes induce learning and memory deficits, and the α isoform is altered in Alzheimer’s Disease2.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 24/01/2021

Anti-Beta 2 Na+/K+ ATPase (extracellular) Antibody (#ANP-012) is a highly specific antibody directed against an epitope of the rat ATP1B2. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope, and could potentially be used for detecting the protein in living cells. It has been designed to recognize ATP1B2 from rat, mouse, and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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