Anti-Human C5aR2 (extracellular) Antibody

C5a anaphylatoxin chemotactic receptor 2, C5a anaphylatoxin receptor 2, Complement component 5a receptor 2, G-protein coupled receptor 77, C5L2, GPR77
    Cat #: AAR-033
    Alternative Name C5a anaphylatoxin chemotactic receptor 2, C5a anaphylatoxin receptor 2, Complement component 5a receptor 2, G-protein coupled receptor 77, C5L2, GPR77
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h
    Immunogen
    • Peptide (C)DSVSYEYGDYSDLSD, corresponding to amino acid residues 4-18 of human C5a anaphylatoxin chemotactic receptor 2 (Accession Q9P296). Extracellular, N-terminus.
    • Anti-Human C5aR2 (extracellular) Antibody
    Accession (Uniprot) Number Q9P296
    Gene ID 27202
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Human only.
    RRID AB_2756542.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Specificity Will not recognize C5a Anaphylatoxin Chemotactic Receptor 2 from rat or mouse samples.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.85 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 2 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic*, ifc*, ih*, ip*, lci*
    Western blot
    • Anti-Human C5aR2 (extracellular) Antibody
      Western blot analysis of human chronic myelogenous leukemia cell lines lysate: MEG-O1 (lanes 1 and 3) and K-562 (lanes 2 and 4):
      1,2. Anti-Human C5aR2 (extracellular) Antibody (#AAR-033), (1:400).
      3,4. Anti-Human C5aR2 (extracellular) Antibody, preincubated with the negative control antigen.
    References
    1. Monk, P.N. et al. (2007) Br. J. Pharmacol. 152, 429.
    Scientific background

    Complement fragment C5a is a pro-inflammatory polypeptide produced during activation of the complement cascade of serum proteins in response to foreign surfaces such as microorganisms and tissue damaged by physical or chemical injury. C5a binds to at least two seven-transmembrane domain receptors, C5aR (C5R1, CD88) and C5L2 (gpr77).

    Expression of C5L2 (C5a Anaphylatoxin Receptor 2) can be found in various tissues and cells such as astrocytes, neutrophils/macrophages, mast cells, immature dendritic cells, as well as in the brain, lung, heart, kidney, liver, ovary or testis.

    The C5L2 receptor is a G-protein coupled receptor clustered with other chemoattractant receptors, such as type-II angiotensin-II receptorbradykinin receptors, the formyl peptide receptor family, ChemR23 and several orphan GPCRs. A small number of single-nucleotide polymorphisms (SNP) have been found in the receptor gene. C5L2 has two synonymous SNPs at 614G/A and 860C, however there are no known associations between these SNPs and human disease.

    Although C5L2 has the conventional structure of a GPCR, studies have shown that C5L2 does not couple to G proteins owing to the lack of a highly conserved Asp-Arg-Tyr motif, found in the third TM domain1.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Anti-Human C5aR2 (extracellular) Antibody (#AAR-033) is a highly specific antibody directed against an epitope of human C5a anaphylatoxin receptor 2 (C5aR2, C5L2). The antibody can be used in western blot analysis. It recognizes an extracellular epitope and is likely to detect the receptor in living cells. It has been designed to recognize C5L2 from human samples only.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
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