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Anti-CaBP2 Antibody

Calcium-binding protein 2
Cat #: ACS-003
Alternative Name Calcium-binding protein 2
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide CAKTPWH RGSKER, corresponding to amino acid residues 4-16 of mouse CaBP2 (Accession Q9JLK4). Intracellular, N-terminus.
Accession (Uniprot) Number Q9JLK4
Gene ID 29866
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat – 12/13 amino acid residues identical; human – 9/13 amino acid residues identical.
RRID AB_2340927.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.85 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ih*, ip*
Western blot
  • Western blot analysis of ARPE-19 (lanes 1 and 4), mouse heart (lanes 2 and 5) and rat heart (lanes 3 and 6) lysates:
    Western blot analysis of ARPE-19 (lanes 1 and 4), mouse heart (lanes 2 and 5) and rat heart (lanes 3 and 6) lysates:
    1-3. Anti-CaBP2 Antibody (#ACS-003), (1:400).
    4-6. Anti-CaBP2 Antibody, preincubated with CaBP2 Blocking Peptide (#BLP-CS003).
References
  1. Park, S. et al. (2011) Protein Sci. 20, 1356.
  2. Li, C. et al. (2009) J. Biol. Chem. 284, 2472.
  3. Findeisen, F. et al. (2010) Structure 18, 1617.
  4. Kinoshita-Kawada, M. et al. (2005) Pflugers Arch. 450, 345.
  5. Schrauwen, I. et al. (2012) Am. J. Hum. Genet. 91, 636.
Scientific background

Neuronal Ca2+-binding proteins (CaBP1-5) are a subclass of the calmodulin (CaM) superfamily that regulates specific Ca2+ channel targets in the brain and retina. Multiple isoforms of CaBPs are localized in different neuronal cell types and perform specialized roles in sensory transduction and disease processes1.

CaBP1–5 proteins have four EF hands that form pairs within the N lobe (EF1 and 2) and C lobe (EF3 and 4). The two lobes are structurally independent and connected by a flexible linker. Whereas all four EF hands bind Ca2+ in CaM, EF2 in CaBP1 does not bind Ca2+, and EF1 has reduced selectivity for Ca2+ over Mg2+. EF3 and EF4 in the C lobe of CaBP1 exhibit canonical Ca2+-induced conformational changes2. CaBP-target interactions induce functional changes distinct from those caused by CaM and may diversify neuronal responses to Ca2+ signals. CaBPs interact with and reshape the functional properties of certain voltage-gated Ca2+ channels (CaVs)3.

CaBP1 is the best characterized family member and has been shown to regulate inositol 1,4,5-triphosphate receptors (IP3Rs), P/Q-type voltage-gated Ca2+ channels, L-type Ca2+ channels, and the transient receptor potential channel (TRPC5)4. CaBP1 regulates the currents of CaV1 and CaV2 channels in neurons, photoreceptors, and auditory hair cells. CaBP interactions with CaV1 channels may be required for hearing and vision, as mutations that disrupt these interactions cause blindness and deafness5.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of mouse Calcium-binding protein 2. Anti-CaBP2 Antibody (#ACS-003) can be used in western blot analysis. It has been designed to recognize CaBP2 from rat, mouse and human samples.

For research purposes only, not for human use
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