Anti-CaBP4 Antibody

Calcium-binding protein 4
    Cat #: ACS-004
    Alternative Name Calcium-binding protein 4
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
    May also work in: h*
    Immunogen
    • Peptide (C)RTYGPLLNRMFGKDR, corresponding to amino acid residues 105-120 of mouse CaBP4 (Accession Q8VHC5). Intracellular.
    • Anti-CaBP4 Antibody
    Accession (Uniprot) Number Q8VHC5
    Gene ID 73660
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat - identical; human - 14/15 amino acid residues identical.
    RRID AB_2340928.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic*, ih*, ip*
    Western blot
    • Anti-CaBP4 Antibody
      Western blot analysis of rat eye lysate:
      1. Anti-CaBP4 Antibody (#ACS-004), (1:200).
      2. Anti-CaBP4 Antibody, preincubated with the negative control antigen.
    References
    1. Haeseleer, F. et al. (2004) Nat. Neurosci. 7, 1079.
    2. Park, S. et al. (2014) Biomol NMR Assign. 8, 361.
    3. Maeda, T. et al. (2005) Invest. Ophthalmol. Vis. Sci. 46, 4320.
    4. Haeseleer, F. et al. (2002) Biochem. Biophys. Res. 290, 615.
    5. Zeitz, C. et al. (2006) Am. J. Hum. Genet. 79, 657.
    Scientific background

    Neuronal calcium-binding proteins (CaBP1-8) belong to a subclass of the calmodulin (CaM) superfamily and regulate in particular Ca2+ channel targets in the brain and retina1.

    CaBP4 contains two globular domains (N- and C-lobe) that each contains a pair of EF- hand motifs connected by a central linker. The second EF-hand in CaBP4 lacks conserved residues in the binding loop and is predicted to not bind Ca2+. CaBP4 interacts structurally with the C-terminal region of CaV1.4 and this Ca2+-dependent interaction has been implicated in the modulation of voltage-dependent CaV1.4 activation2.

    Multiple isoforms of CaBPs are localized in different neuronal cell types and perform specialized roles in signal transduction. The CaBP4 isoform regulates voltage-gated Ca2+ channels in retinal photoreceptor cells. In the eye, CaBP4 is localized at the photoreceptor synaptic terminals in rods and cones3. CaBP4 is essential for the development and maintenance of the photoreceptor synapse, likely through functional modulation of the photoreceptor Ca2+ channels, α1F L-type voltage- dependent calcium channels (CaV1.4), and neurotransmitter release4.

    Mutations in both CaV1.4 and CaBP4 have been identified in patients suffering from congenital stationary night blindness type 2 (CSNB2), indicating that defects in Ca2+-dependent regulation of CaV1.4 by CaBP4 are closely associated with CSNB2. Indeed, deletion of CaBP4 in the mouse leads to a CSNB2-like phenotype5.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of mouse Calcium-binding protein 4. Anti-CaBP4 Antibody (#ACS-004) can be used in western blot analysis. It has been designed to recognize CaBP4 from rat, mouse and human samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
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