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Anti-CatSper4 (extracellular) Antibody

Cation channel sperm-associated protein 4
Cat #: ACC-304
Alternative Name Cation channel sperm-associated protein 4
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide (C)NSYLGQKHYELFSTID, corresponding to amino acid residues 90-105 of mouse CatSper4 (Accession Q8BVN3). 1st extracellular loop.
Accession (Uniprot) Number Q8BVN3
Gene ID 329954
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat - identical; human -15/16 amino acid residues identical.
RRID AB_2340919.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, wb
May also work in: ifc*, ih*, ip*, lci*
Western blot
  • Western blot analysis of rat testis lysate:
    Western blot analysis of rat testis lysate:
    1. Anti-CatSper4 (extracellular) Antibody (#ACC-304), (1:1000).
    2. Anti-CatSper4 (extracellular) Antibody preincubated with CatSper4 (extracellular) Blocking Peptide (#BLP-CC304).
Immunocytochemistry
  • Macaque sperm cells (1:100) (Sumigama, S. et al. (2015) Biol. Reprod. 93, 130.).
References
  1. Yanagimachi, R. et al. (1994) The Physiology of Reproduction (Raven Press, New York) 2nd edition, 189.
  2. Jin, J.L. et al. (2005) Biol. Reprod. 73, 1235.
  3. Quill, T.A. et al. (2001) Proc. Natl. Acad. Sci. U.S.A. 98, 12527.
  4. Ren, D. et al. (2001) Nature 413, 603.
  5. Kirichok, Y. et al. (2006) Nature 439, 737.
  6. Lobley, A. et al. (2003) Reprod. Biol. Endocrinol. 1, 53.
  7. Carlson, A.E. et al. (2005) J. Biol. Chem. 280, 32238.
Scientific background

During mammalian fertilization, Ca2+ signaling plays a central role in sperm capacitation and motility to the fusion between sperm and egg1. Several Ca2+-permeable ion channel proteins have been characterized in mammalian sperm. However, only the four mammalian CatSper members (CatSpers 1-4) are restrictively expressed in the testis and are clearly shown to be required for male fertility2.

CatSper channels are named after the first putative cation channel of sperm3. CatSper4 contains a single ion transport domain comprised of 6 transmembrane spanning regions, where the fourth transmembrane region resembles a voltage sensor and a pore forming region lies between transmembrane regions 5 and 6. The pore contains the consensus sequence T×D×W indicative of a probable Ca2+ selective channel4. The four CatSper proteins are primarily localized to the plasma membrane of the distal portion of the sperm tail and presumably form a heterotetrameric, pH- and voltage-dependent Ca2+-permeable channel 5
CatSper 4 is highly expressed in testis and expressed at low levels in placenta and lung6.

Male mice deficient in any of the four CatSper are completely sterile but exhibit no other apparent abnormality. The major phenotypes of the null sperm are a lack of hyperactivated motility, a gradual loss of motility after isolation, and an inability to penetrate zona-intact eggs7.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of mouse CatSper4. Anti-CatSper4 (extracellular) Antibody (#ACC-304) can be used in western blot and immunocytochemistry applications. It has been designed to recognize CatSper4 from rat, mouse and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Immunocytochemistry citations
  1. Macaque sperm cells (1:100).
    Sumigama, S. et al. (2015) Biol. Reprod. 93, 130.
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