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Anti-CaV1.3 (CACNA1D) (extracellular) Antibody

Voltage-dependent L-type calcium channel subunit α1D

Cat #: ACC-311
Alternative Name Voltage-dependent L-type calcium channel subunit α1D
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)EQLTKETEGGNHS, corresponding to amino acid residues 215-227 of rat CaV1.3 (Accession P27732). 2nd extracellular loop, repeat I.
Accession (Uniprot) Number P27732
Gene ID 29716
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human - identical.
RRID AB_2340920.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.9 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ifc, ih, lci, wb
May also work in: ip*
Western blot
  • Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5), and rat C6  brain Glioma (lanes 3 and 6) lysates:
    Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5), and rat C6  brain Glioma (lanes 3 and 6) lysates:
    1-3. Anti-CaV1.3 (CACNA1D) (extracellular) Antibody (#ACC-311), (1:200).
    4-6. Anti-CaV1.3 (CACNA1D) (extracellular) Antibody, preincubated with Cav1.3/CACNA1D (extracellular) Blocking Peptide (#BLP-CC311).
Immunohistochemistry
  • Expression of CaV1.3 in rat DRG
    Expression of CaV1.3 in rat DRG
    Immunohistochemical staining of adult rat dorsal root ganglion (DRG) using Anti-CaV1.3 (CACNA1D) (extracellular) Antibody (#ACC-311). A. CaV1.3 labeling (red) appears in the cell bodies of the DRG. B. Nuclear staining using DAPI as the counterstain. C. Merged image of A and B.
Indirect flow cytometry
  • Cell surface detection of Cav1.3 by indirect flow cytometry in live intact human Jurkat T-cell leukemia cells: 
    Cell surface detection of Cav1.3 by indirect flow cytometry in live intact human Jurkat T-cell leukemia cells: 
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-CaV1.3 (CACNA1D) (extracellular) Antibody (#ACC-311), (5μg) + goat-anti-rabbit-FITC.
Live cell imaging / Immunocytochemistry
  • Expression of CaV1.3 in rat PC12 cells
    Expression of CaV1.3 in rat PC12 cells
    Cell surface detection of CaV1.3 in intact living rat pheochromocytoma (PC12) cells. A. Extracellular staining of cells using Anti-CaV1.3 (CACNA1D) (extracellular) Antibody (#ACC-311), (1:50), (red). B. Merge of A with the live view of the cell.
References
  1. Catterall, W.A. et al. (2003) Pharmacol. Rev. 55, 579.
  2. IUPHAR
  3. Mangoni, M.E. et al. (2003) Proc. Natl. Acad. Sci. U.S.A. 100, 5543.
  4. Zhang, Z. et al. (2005) Circulation 112, 1936.
Scientific background

All L-type calcium channels are encoded by one of the CaV1 channel genes. These channels play a major role as a Ca2+ entry pathway in skeletal cardiac and smooth muscles as well as in neurons, endocrine cells and possibly in non-excitable cells such as hematopoetic and epithelial cells. All CaV1 channels are influenced by dihydropyridines (DHP) and are also referred to as DHP receptors. While the CaV1.1 and CaV1.4 isoforms are expressed in restricted tissues (skeletal muscle and retina, respectively), the expression of CaV1.2 is ubiquitous.1,2

The CaV1.3 channels are also expressed, as are other L-type channels, in neurons and neuroendocrine cells. However, accumulated data has shown the expression of CaV1.3 in heart and suggests that it plays a major role in the generation of cardiac pacemaker activity.3,4

Several peptidyl toxins have been described that are specific L-type channel blockers. These include the Mamba toxins Calcicludine (#SPC-650), Calciseptine (#C-500) and FS-2 (#F-700). So far no selective blocker for one of the CaV1 isoforms has been described.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Lyophilized Powder
Last update: 02/01/2022

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the rat protein. Anti-CaV1.3 (CACNA1D) (extracellular) Antibody (#ACC-311) can be used in western blot and immunohistochemistry and live cell imaging applications. It is specially suited to detect CaV1.3 channel in live cells. It has been designed to recognize CaV1.3 from mouse, rat and human samples.

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For research purposes only, not for human use

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