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Anti-CaV1.3 (CACNA1D) (extracellular) Antibody

Voltage-dependent L-type calcium channel subunit α1D
    Cat #: ACC-311
    Alternative Name Voltage-dependent L-type calcium channel subunit α1D
  • Lyophilized Powder
  • Antigen Incl.
    • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)EQLTKETEGGNHS, corresponding to amino acid residues 215-227 of rat CaV1.3 (Accession P27732). 2nd extracellular loop, repeat I.
    • Anti-CaV1.3 (CACNA1D) (extracellular) Antibody
    Accession (Uniprot) Number P27732
    Gene ID 29716
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human - identical.
    RRID AB_2340920.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.9 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, lci, wb
    May also work in: ifc*, ip*
    Western blot
    • Anti-CaV1.3 (CACNA1D) (extracellular) Antibody
      Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and C6 (lanes 3 and 6) lysates:
      1-3. Anti-CaV1.3 (CACNA1D) (extracellular) Antibody (#ACC-311), (1:200).
      4-6. Anti-CaV1.3 (CACNA1D) (extracellular) Antibody, preincubated with the negative control antigen.
    Immunohistochemistry
    • Anti-CaV1.3 (CACNA1D) (extracellular) Antibody
      Expression of CaV1.3 in rat DRG
      Immunohistochemical staining of adult rat dorsal root ganglion (DRG) using Anti-CaV1.3 (CACNA1D) (extracellular) Antibody (#ACC-311). A. CaV1.3 labeling (red) appears in the cell bodies of the DRG. B. Nuclear staining using DAPI as the counterstain. C. Merged image of A and B.
    Live cell imaging / Immunocytochemistry
    • Anti-CaV1.3 (CACNA1D) (extracellular) Antibody
      Expression of CaV1.3 in rat PC12 cells
      Cell surface detection of CaV1.3 in intact living rat pheochromocytoma (PC12) cells. A. Extracellular staining of cells using Anti-CaV1.3 (CACNA1D) (extracellular) Antibody (#ACC-311), (1:50), (red). B. Merge of A with the live view of the cell.
    References
    1. Catterall, W.A. et al. (2003) Pharmacol. Rev. 55, 579.
    2. IUPHAR
    3. Mangoni, M.E. et al. (2003) Proc. Natl. Acad. Sci. U.S.A. 100, 5543.
    4. Zhang, Z. et al. (2005) Circulation 112, 1936.
    Scientific background

    All L-type calcium channels are encoded by one of the CaV1 channel genes. These channels play a major role as a Ca2+ entry pathway in skeletal cardiac and smooth muscles as well as in neurons, endocrine cells and possibly in non-excitable cells such as hematopoetic and epithelial cells. All CaV1 channels are influenced by dihydropyridines (DHP) and are also referred to as DHP receptors. While the CaV1.1 and CaV1.4 isoforms are expressed in restricted tissues (skeletal muscle and retina, respectively), the expression of CaV1.2 is ubiquitous.1,2

    The CaV1.3 channels are also expressed, as are other L-type channels, in neurons and neuroendocrine cells. However, accumulated data has shown the expression of CaV1.3 in heart and suggests that it plays a major role in the generation of cardiac pacemaker activity.3,4

    Several peptidyl toxins have been described that are specific L-type channel blockers. These include the Mamba toxins Calcicludine (#SPC-650), Calciseptine (#C-500) and FS-2 (#F-700). So far no selective blocker for one of the CaV1 isoforms has been described.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Lyophilized PowderNegative Control Antigen Included
    receive a 25 µl free trial sample!
    Last update: 24/01/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the rat protein. Anti-CaV1.3 (CACNA1D) (extracellular) Antibody (#ACC-311) can be used in western blot and immunohistochemistry and live cell imaging applications. It is specially suited to detect CaV1.3 channel in live cells. It has been designed to recognize CaV1.3 from mouse, rat and human samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations

    Related Products

    Shipping and Ordering information

    Resources

    Negative Control Antigen Manipulation

    L-Type (CaV1) Channels

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