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Anti-CaV2.3 (CACNA1E) Antibody

CACH6, CACNL1A6, Voltage-dependent R-type calcium channel subunit α1E, BII, Brain calcium channel II, RBE2
Cat #: ACC-006
Alternative Name CACH6, CACNL1A6, Voltage-dependent R-type calcium channel subunit α1E, BII, Brain calcium channel II, RBE2
  • KO Validated
  • Lyophilized Powder yes
    Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide (C)SASQERSLDEGVSIDG, corresponding to amino acid residues 892-907 of rat CaV2.3 (Accession Q07652). Intracellular loop between domains II and III.
    Accession (Uniprot) Number Q07652
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - 14/16 amino acid residues identical; rabbit - 12/16 amino acid residues identical; human - 11/16 amino acid residues identical.
    RRID AB_2039777.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.3 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, wb
    May also work in: ifc*, ip*
    Western blot
    • Western blot analysis of rat brain membranes:
      Western blot analysis of rat brain membranes:
      1. Anti-CaV2.3 (CACNA1E) Antibody (#ACC-006), (1:200).
      2. Anti-CaV2.3 (CACNA1E) Antibody, preincubated with Cav2.3/CACNA1E Blocking Peptide (#BLP-CC006).
    Immunohistochemistry
    • Expression of CaV2.3 in mouse cerebellum
      Expression of CaV2.3 in mouse cerebellum
      Immunohistochemical staining of mouse cerebellum with Anti-CaV2.3 (CACNA1E) Antibody (#ACC-006). CaV2.3 (red) appears in both soma and dendritic trees of several Purkinje cells. Staining of neurofilament 200 (green) in the same brain section demonstrates the relationship between axons passing through the granule layer and the Purkinje cells.
    Immunocytochemistry
    • Mouse spiral ganglion (1:50) (Chen, W.C. et al. (2011) Hear. Res. 278, 52.).

      Human sperm cells (1:100) (Trevino, C.L. et al. (2004) FEBS Lett. 563, 87.).
    References
    1. Tsunemi, T. et al. (2002) J. Biol. Chem. 277, 7214.
    2. Gasparini, S. et al. (2001) J. Neurosci.21, 8715.
    3. Lanzafame, A.A. et al. (2003) Receptors Channels 9, 241.
    Scientific background

    Voltage-dependent Ca2+ channels (CaV channels) are pivotal players in many physiological roles such as secretion, contraction, migration and excitation.1

    The voltage-dependent Ca2+ channels are composed of several subunits; α1, β, α2δ and γ. CaV channels were originally divided into six physiological types: L-, N-, P-, Q-, R-, and T-type.1

    The CaV2.3 (formerly named α1E) protein, forms the R-type channels. R-type channels are highly sensitive to both SNX-482 (#RTS-500) and NiCl2. CaV2.3 channels were shown to mediate fast excitatory transmission in several synapses including the hippocampus.2

    CaV2.3 channels can be modulated by the muscarinic G-protein coupled receptors. 

    In HEK-293 cells, expressing CaV2.3 channels, the R-type channel was shown to be both facilitated and inhibited by m1 and m2 muscarinic receptors. However, inhibition was much weaker than facilitation for the m1 receptor, while for m2 receptors, inhibition was much more pronounced than activation.3

    Facilitation and inhibition by the same receptors was probably exerted via different pathways as was indicated by their response to various inhibitors.3

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title: Anti-Cav2.3 (CACNA1E) AntibodyExpression of CaV α subunits in rat ICG.Immunohistochemical staining of rat intracardiac ganglia (ICG) using Anti-CaV1.2 (CACNA1C) Antibody (#ACC-003), Anti-CaV1.3 (CACNA1D) Antibody (#ACC-005), Anti-CACNA1A (CaV2.1) Antibody (#ACC-001), Anti-CACNA1B (CaV2.2) Antibody (#ACC-002) and Anti-CaV2.3 (CACNA1E) Antibody (#ACC-006). All CaV subtypes are expressed in sham treated rats but N-type CaV channel levels are decreased in CHF rats.Adapted from Tu, H. et al. (2014) with permission of the American Physiological Society.
    Last update: 16/08/2020

    Alomone Labs is pleased to offer an antibody raised against an epitope of the rat CaV2.3 channel. Anti-CaV2.3 (CACNA1E) Antibody (#ACC-006) can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize CaV2.3 from human, mouse and rat samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    KO validation citations
    1. Western blot analysis of mouse brain lysate. Tested in CaV2.3-/- mice.
      Saegusa, H. et al. (2000) Proc. Natl. Acad. Sci. U.S.A. 97, 6132.
    Western blot citations
    1. Mouse brain lysate. Tested in CaV2.3-/- mice.
      Saegusa, H. et al. (2000) Proc. Natl. Acad. Sci. U.S.A. 97, 6132.
    Immunohistochemistry citations
    1. Mouse olfactory bulb sections (1:100).
      Gorin, M. et al. (2016) J. Neurosci. 36, 3127.
    2. Rat intracardiac ganglia and stellate ganglia neurons.
      Tu, H. et al. (2014) Am. J. Physiol. 306, C132.
    Immunocytochemistry citations
    1. Mouse spiral ganglion (1:50).
      Chen, W.C. et al. (2011) Hear. Res. 278, 52.
    2. Human sperm cells (1:100).
      Trevino, C.L. et al. (2004) FEBS Lett. 563, 87.
    More product citations
    1. Rodriguez-Tapia, E.S. et al. (2017) Exp. Physiol. 102, 299.
    2. Shakeri, B. et al. (2012) J. Biol. Chem. 287, 32835.
    3. Ohta, T. et al. (2010) Biochem. Biophys. Res. Commun. 394, 464.
    4. Murakami, M. et al. (2007) Biochem. Biophys. Res. Commun. 354, 1016.
    5. Shiraiwa, T. et al. (2007) Biochem. Biophys. Res. Commun. 355, 1019.
    6. Fisher, T.E. et al. (2000) FEBS Lett. 482, 131.
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