Anti-CACNB2 Antibody

CaVβ2, Voltage-dependent L-type calcium channel subunit beta-2, CACNLB2, CAB2, CACB2, Lambert-Eaton myasthenic syndrome antigen B, MYSB
    Cat #: ACC-105
    Alternative Name CaVβ2, Voltage-dependent L-type calcium channel subunit beta-2, CACNLB2, CAB2, CACB2, Lambert-Eaton myasthenic syndrome antigen B, MYSB
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
    May also work in: h*
      • Peptide (C)HEHVDRYVPHREHNHRE, corresponding to amino acid residues 571-587 of rat CACNB2 (Accession Q8VGC3). Intracellular, C-terminus.
        Anti-CACNB2 Antibody
    Accession (Uniprot) Number Q8VGC3
    Gene ID 116600
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human - 11/17 amino acid residues identical.
    RRID AB_2340905.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.85 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic*, ifc*, ih*, ip*
      • Anti-CACNB2 Antibody
        Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:
        1,2. Anti-CACNB2 Antibody (#ACC-105), (1:800).
        3,4. Anti-CACNB2 Antibody, preincubated with the negative control antigen.
    References
      • Voltage-dependent Ca2+ channels are a family of membrane proteins that allow cells to couple electrical activity to intracellular Ca2+ signaling1. Voltage-gated Ca2+ channels are classified as T, L, N, P, Q and R, and are distinguished by their sensitivity to pharmacological blocks, single-channel conductance kinetics, and voltage-dependence.

        On the basis of their voltage activation properties, voltage-gated Ca2+ subtypes can be further divided into two broad groups: the low (T-type) and high (L, N, P, Q and R-type) threshold-activated channels2. The activity of the channel pore is modulated by 4 tightly-coupled subunits: an intracellular β subunit; a transmembrane γ subunit; and a disulphide-linked complex of α2 and δ subunits3. There are four distinct β subunits: β1, β2, β3 and β44. There are 4 splice variants of the β2 subunit: β2a, β2b, β2c and β2d. β2a and β2b are expressed in heart, aorta and brain, and are 606- and 632-residue proteins, respectively. β2c and β2d (655 and 604 residues, respectively) are expressed in brain, and recent studies show beta-2c to be also expressed in heart. All splice variants differ in their N-terminal regions5.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 01/09/2019

    Anti-CACNB2 Antibody (#ACC-105) is a highly specific antibody directed against an epitope of rat CaVβ2. The antibody can be used in western blot analysis. It has been designed to recognize CaVβ2 from rat, mouse, and human samples.

    For research purposes only, not for human use
    Citations
      • Rat cardiomyocytes.
        Moreno, C. et al. (2015) Pflugers Arch. 467, 2473.
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