Anti-CACNA2D1 (CaVα2δ1) (extracellular) Antibody

Voltage-dependent Ca2+ channel subunit alpha-2/delta-1, L-type calcium channel alpha 2 polypeptide, Dihydropyridine-sensitive L-type calcium channel alpha-2/delta subunit
    Cat #: ACC-015
    Alternative Name Voltage-dependent Ca2+ channel subunit alpha-2/delta-1, L-type calcium channel alpha 2 polypeptide, Dihydropyridine-sensitive L-type calcium channel alpha-2/delta subunit
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, r
    May also work in: m*
      • Peptide EPFPSAVTIKSWVDK(C), corresponding to amino acid residues 27-41 of rabbit CaVα2δ1 precursor (Accession P13806). Extracellular, N-terminus.
        Anti-CACNA2D1 (CaVα2δ1) (extracellular) Antibody
    Accession (Uniprot) Number P13806
    Gene ID 100009105
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Pig, human - identical; mouse, rat - 14/15 amino acid residues identical.
    RRID AB_2039785.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.65 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 0.75 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, lci, wb
    May also work in: ifc*, ih*, ip*
      • Anti-CACNA2D1 (CaVα2δ1) (extracellular) Antibody
        Western blot analysis of rat brain membranes:
        1. Anti-CACNA2D1 (CaVα2δ1) (extracellular) Antibody (#ACC-015), (1:200).
        2. Anti-CACNA2D1 (CaVα2δ1) (extracellular) Antibody, preincubated with the negative control antigen.
      • Rat primary spinal neurons (Favereaux, A. et al. (2011) EMBO J. 30, 3830.).
      • Anti-CACNA2D1 (CaVα2δ1) (extracellular) Antibody
        Expression of CaVα2δ1 in rat PC12 cells
        Cell surface detection of CaVα2δ1 in intact living rat Pheochromocytoma (PC12) cells. A. Extracellular staining of cells using Anti-CACNA2D1 (CaVα2δ1) (extracellular) Antibody (#ACC-015), (1:50), (red). B. Merge of A with the live view of the cell.
    References
    • 1. Davis, A. et al. (2007) Trends Pharm. Sci. 28, 220.
    • 2. Singer, D. et al. (1991) Science 253, 1553.
    • 3. Field, M.J. et al. (2006) Proc. Natl. Acad. Sci. U.S.A. 103, 17537.
      • The α2δ1 protein, encoded by CACNA2D1, is an auxiliary subunit of the CaV (voltage-dependent calcium) channel multimer complex. Four α2δ isoforms were identified in mammalian genomes. They share some sequence, as well as structure, homology.1

        The protein product of the gene encoding α2δ is complex; it includes two separated proteins - the extracellular α2 peptide linked by disulfide bridges to the membrane spanning (single transmembrane helix) δ subunit.

        The α2δ1 subunit is highly expressed in skeletal muscle and brain. Its expression along with a pore forming CaV αsubunit, result in larger CaV currents, probably due to improved trafficking of the α1 subunit to the plasma membrane.1-2 α2δsubunit was shown to be the target of the antiepileptic and analgesic drug Gabapentin.3

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 31/12/2019

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rabbit CaVα2δ1. Anti-CACNA2D1 (CaVα2δ1) (extracellular) Antibody (#ACC-015) can be used in western blot and live cell imaging applications. It is especially suited to detect CaVα2δ1 in live cells. It has been designed to recognize CaVα2δ1 from rat, mouse and human samples.

    For research purposes only, not for human use
    Citations
      • Rat DRG and spinal cord lysates.
        Chen, Y. et al. (2019) J. Neurochem. 148, 252.
      • Mouse spinal cord and small intestine lysates.
        Akamine, T. et al. (2015) J. Pharmacol. Exp. Ther. 354, 65.
      • Transfected HEK 293 cell lysates (1:500).
        Bourdin, B. et al. (2015) J. Biol. Chem. 290, 2854.
      • Mouse brain lysates (1:1000).
        Cordeira, J.W. et al. (2014) J. Neurosci. 34, 554.
      • Rat primary spinal neurons.
        Favereaux, A. et al. (2011) EMBO J. 30, 3830.
    Related Products