Anti-CACNA2D3 (CaVα2δ3) (extracellular)-ATTO-594 Antibody

Voltage-dependent calcium channel subunit alpha-2/delta-3
Cat #: ACC-103-AR
Alternative Name Voltage-dependent calcium channel subunit alpha-2/delta-3
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
      • Peptide CSWWHSDMTAKAQK, corresponding to amino acid residues 942-955 of rat CaVα2δ3 (Accession Q8CFG5). Extracellular.
    Accession (Uniprot) Number Q8CFG5
    Gene ID 306243
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human - identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Label ATTO-594. Maximum absorption 601 nm; maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label is related to the Rhodamine dyes and can be used with filters used to detect Texas Red and Alexa-594.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #ACC-103), and immunocytochemistry (labeled antibody).
    Applications: ic, ih, lci
      • Expression of CaVα2δ3 in rat hippocampus and cortex
        Immunohistochemical staining of rat hippocampal CA3 region (A) and rat neocortex (B) using Anti-CACNA2D3 (CaVα2δ3) (extracellular)-ATTO-594 Antibody (#ACC-103-AR). In both, A and B, CaVα2δ3 staining (red) appears in pyramidal neurons (arrows). DAPI is used as the counterstain (blue).
      • Expression of CaVα2δ3 in PC12 cells
        Cell surface detection of CaVα2δ3 in intact living rat PC12 cells. Extracellular staining of cells using Anti-CACNA2D3 (CaVα2δ3) (extracellular)-ATTO-594 Antibody (#ACC-103-AR), (1:25).
      • Voltage-gated Ca2+ (CaV) channels are ubiquitously expressed and function as Ca2+ conducting pores in the plasma membrane1. On the basis of their voltage activation properties, CaV channels can be further divided into two broad groups: the low (T-type) and high (L, N, P, Q and R-type) threshold-activated channels2. HVA channels are heteromultimers composed of four independently encoded proteins, the pore-forming α1 subunit, which triggers Ca2+ flow across the membrane, and the auxiliary subunits α2δ, γ, and β3. The Ca2+ channel α2δ subunit is a heavily glycosylated protein that is encoded by a single gene and post-translationally cleaved to yield α2 and δ subunits linked by a disulfide bond with a single transmembrane segment4.  The α2δ subunit regulates many functional aspects of Ca2+ channels, such as gating, regulating voltage dependent kinetics, and increasing functional channel density on the plasma membrane5.

        There are four proteins that comprise CaVα2δ: CaVα2δ1, CaVα2δ2, CaVα2δ3 and CaVα2δ46. The CaVα2δ3 subunit is predominantly expressed in neuronal tissue. The CaVα2δ3 subunit regulates all classes of HVA calcium channels. The Caα2δ3 subunits in the nerve terminal function in synaptic morphogenesis and cytoskeletal organization, and that this role is independent of their function in α1 subunit localization and physiology.

        CaVα2δ3 is likely to be the primary presynaptic α2δ isoform mediating morphological development of the neuromuscular junction (NMJ), since null alleles have such a large effect on NMJ development and abolish all action-potential evoked transmission7. Recent study shows that methylation-dependent transcriptional silencing of CaVα2δ3 may contribute to the metastatic phenotype of breast cancer8.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 04/06/2019

    Anti-CACNA2D3 (CaVα2δ3) (extracellular) Antibody (#ACC-103) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize CaVα2δ3 from rat, mouse and human samples.

    Anti-CACNA2D3 (CaVα2δ3) (extracellular)-ATTO-594 Antibody (#ACC-103-AR) is directly labeled with an ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-CACNA2D3 (CaVα2δ3) (extracellular)-ATTO-594 Antibody has been tested in immunocytochemistry and immunohistochemistry applications and is especially suited for experiments requiring simultaneous labeling of different markers.

    For research purposes only, not for human use