- Peptide CSWWHSDMTAKAQK, corresponding to amino acid residues 942-955 of rat CaVα2δ3 (Accession Q8CFG5). Extracellular.
- Western blot analysis of rat brain (lanes 1 and 3) and human K562 chronic myelogeneous leukemia (lanes 2 and 4) lysates:1,2. Anti-CACNA2D3 (CaVα2δ3) (extracellular) Antibody (#ACC-103), (1:200).
3,4. Anti-CACNA2D3 (CaVα2δ3) (extracellular) Antibody, preincubated with CACNA2D3/Cavα2δ3 (extracellular) Blocking Peptide (#BLP-CC103).
- Expression of CaVα2δ3 in rat hippocampus and cortexImmunohistochemical staining of rat hippocampal CA3 region (A) and rat neocortex (B) using Anti-CACNA2D3 (CaVα2δ3) (extracellular) Antibody (#ACC-103). In both areas CaVα2δ3 staining (red) appears in pyramidal neurons (arrow). DAPI is used as the counterstain (blue).
- Expression of CaVα2δ3 in rat PC12 cellsCell surface detection of CaVα2δ3 in intact living PC12 cells. Extracellular staining of cells using Anti-CACNA2D3 (CaVα2δ3) (extracellular) Antibody (#ACC-103), (1:25) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody.
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Voltage-gated Ca2+ (CaV) channels are ubiquitously expressed and function as Ca2+ conducting pores in the plasma membrane1. On the basis of their voltage activation properties, CaV channels can be further divided into two broad groups: the low (T-type) and high (L, N, P, Q and R-type) threshold-activated channels2. HVA channels are heteromultimers composed of four independently encoded proteins, the pore-forming α1 subunit, which triggers Ca2+ flow across the membrane, and the auxiliary subunits α2δ, γ, and β3. The Ca2+ channel α2δ subunit is a heavily glycosylated protein that is encoded by a single gene and post-translationally cleaved to yield α2 and δ subunits linked by a disulfide bond with a single transmembrane segment4. The α2δ subunit regulates many functional aspects of Ca2+ channels, such as gating, regulating voltage dependent kinetics, and increasing functional channel density on the plasma membrane5.
There are four proteins that comprise CaVα2δ: CaVα2δ1, CaVα2δ2, CaVα2δ3 and CaVα2δ46. The CaVα2δ3 subunit is predominantly expressed in neuronal tissue. The CaVα2δ3 subunit regulates all classes of HVA calcium channels. The Caα2δ3 subunits in the nerve terminal function in synaptic morphogenesis and cytoskeletal organization, and that this role is independent of their function in α1 subunit localization and physiology.
CaVα2δ3 is likely to be the primary presynaptic α2δ isoform mediating morphological development of the neuromuscular junction (NMJ), since null alleles have such a large effect on NMJ development and abolish all action-potential evoked transmission7. Recent study shows that methylation-dependent transcriptional silencing of CaVα2δ3 may contribute to the metastatic phenotype of breast cancer8.
Species reactivity key:
Anti-CACNA2D3 (CaVα2δ3) (extracellular) Antibody (#ACC-103) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize CaVα2δ3 from rat, mouse and human samples.
- Tedeschi, A. et al. (2016) Neuron 92, 419.