Anti-CACNA2D4 (CaVα2δ4) (extracellular) Antibody

Voltage-dependent calcium channel subunit alpha-2/delta-4, RCD4
    Cat #: ACC-104
    Alternative Name Voltage-dependent calcium channel subunit alpha-2/delta-4, RCD4
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
      • Peptide (C)SERPQEMGRLLGEADG, corresponding to amino acid residues 881-896 of mouse CaVα2δ4 (Accession Q5RJF7). Extracellular.
        Anti-CACNA2D4 (CaVα2δ4) (extracellular) Antibody
    Accession (Uniprot) Number Q5RJF7
    Gene ID 319734
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat - 15/16 amino acid residues identical.
    Human - not recommended for use with human samples.
    RRID AB_2340903.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, lci, wb
    May also work in: ifc*, ip*
      • Anti-CACNA2D4 (CaVα2δ4) (extracellular) Antibody
        Western blot analysis of rat DRG (lanes 1 and 3) and PC12 cells (lanes 2 and 4):
        1,2. Anti-CACNA2D4 (CaVα2δ4) (extracellular) Antibody (#ACC-104), (1:200).
        3,4. Anti-CACNA2D4 (CaVα2δ4) (extracellular) Antibody, preincubated with the negative control antigen.
      • Anti-CACNA2D4 (CaVα2δ4) (extracellular) Antibody
        Expression of CaVα2δ4 in rat DRG
        Immunohistochemical staining of rat DRG frozen section using Anti-CACNA2D4 (CaVα2δ4) (extracellular) Antibody (#ACC-104), (1:100). A. CaVα2δ4 is expressed in DRG neurons. B. Nuclear staining using Hoechst 33342 as the counterstain. C. Merged images of A and B.
      • Anti-CACNA2D4 (CaVα2δ4) (extracellular) Antibody
        Expression of CaVα2δ4 in rat C6 cells
        Cell surface detection of CaVα2δ4 in intact living rat brain glioma (C6) cells. A. Extracellular staining of cells with Anti-CACNA2D4 (CaVα2δ4) (extracellular) Antibody (#ACC-104), (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Nuclear staining of cells using the cell-permeable dye Hoechst 33342 (blue). C. Merged images of A and B.
    References
      • Voltage-gated Ca2+ (CaV) channels are ubiquitously expressed and function as Ca2+ conducting pores in the plasma membrane1. Based on their electrophysiological and pharmacological properties, Ca2+ channels have traditionally been classified into L, T, N, P, Q, and R types2. L-type calcium channels are heteromultimers composed of four independently encoded proteins, the pore-forming α1 subunit, which triggers Ca2+ flow across the membrane, and the auxiliary subunits α2δ, γ, and β3. The Ca2+ channel α2δ subunit is a heavily glycosylated protein that is encoded by a single gene and post-translationally cleaved to yield α2 and δ subunits linked by a disulfide bond with a single transmembrane segment4.

        The α2δ subunit regulates many functional aspects of Ca2+ channels, such as gating, regulating voltage dependent kinetics, and increasing functional channel density on the plasma membrane5.  There are four proteins that comprise CaVα2δ: CaVα2δ1CaVα2δ2CaVα2δ3 and CaVα2δ46. CaVα2δ4 participates in the regulation of membrane expression of CaV channels. It is predominantly expressed in certain types of endocrine cells. It is also detected in the erythroblasts in the fetal liver, in the cells of the zona reticularis of the adrenal gland and in the basophiles of the pituitary7. Defects in CaVα2δ4 are the cause of retinal cone dystrophy 4 (RCD4). RCD4 is characterized by minimal symptoms except for slowly progressive reduction in visual acuity8.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry,
    IF- Immunofluorescence, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 10/11/2019

    Anti-CACNA2D4 (CaVα2δ4) (extracellular) Antibody (#ACC-104) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunocytochemistry and immunohistochemistry applications. It is especially suited to detect CaVα2δ4 in living cells. It has been designed to recognize CaVα2δ4 from rat and mouse samples.

    For research purposes only, not for human use
    Citations
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