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Anti-CACNG6 (extracellular) Antibody

CaVγ6, Voltage-dependent calcium channel gamma-6 subunit

Cat #: ACC-112
Alternative Name CaVγ6, Voltage-dependent calcium channel gamma-6 subunit
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide CIKRLWQADVPAGR, corresponding to amino acid residues 87-100 of rat CACNG6 (Accession Q8VHW3).
Accession (Uniprot) Number Q8VHW3
Gene ID 54378
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse – identical; human – 11/14 amino acid residues identical.
RRID AB_2756550.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.85 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*, lci*
Western blot
  • Western blot analysis of rat skeletal muscle lysate:
    Western blot analysis of rat skeletal muscle lysate:
    1. Anti-CACNG6 (extracellular) Antibody (#ACC-112), (1:200).
    2. Anti-CACNG6 (extracellular) Antibody, preincubated with CACNG6 (extracellular) Blocking Peptide (#BLP-CC112).
Immunohistochemistry
  • Expression of CaVγ6 (CACNG6) in rat brain
    Expression of CaVγ6 (CACNG6) in rat brain
    Immunohistochemical staining of rat cingulate cortex using Anti-CACNG6 (extracellular) Antibody (#ACC-112), (1:400). A. CaVγ6 staining (green) appears in the pyramidal layer (arrow). B. Nuclear staining using DAPI as the counterstain. C. Merged images of A and B.
References
  1. Lee, J.S. et al. (2010) BMC Med. Genet. 11, 138.
  2. Thomas, G. et al. (2009) FASEB J. 23, 998.
  3. Chen, R.S. et al. (2007) Cell. Biochem. Biophys. 47, 178.
Scientific background

The calcium channel voltage-dependent gamma subunit 6 encoded by the CACNG6 gene, encodes an integral membrane protein. Its role is to stabilize the calcium channel during the channels inactive state. This subunit is responsible for decreasing Ca2+ currents when it is co-expressed with the low voltage activated Ca2+ channel subunit1,2

The calcium channel γ subunits comprise eight members that share a common topology.
This family is divided in three clusters: The first- γ1 and γ6, second γ5 and γ7 and third γ2,3,4,8. 

CaVγ6 protein is predominantly expressed in striated muscle cells. It is also expressed in skeletal and cardiac muscles and slightly in the brain as a separate isoform.

CaVγ6 structure includes four transmembrane domains (TM) with short intracellular N- and C-terminus. The first TM with a specifically motif- GxxxA is critical for CaVγ6 inhibitory function2. Phylogenetic analysis has suggested that all γ subunits developed from a single ancestral gene through tandem repeat and chromosome duplication3.

Voltage-dependent calcium channels are important regulators of calcium influx and are important for several physiological processes including- synaptic transmission, muscle contraction, neurogenesis, hormone secretion, cell motility, cell division and development.

Several recent studies have shown a negative correlation of CaVγ6 gene expression to chronic obstructive pulmonary disease, responses of the human airway epithelium following injury and parenchyma in lung tissues1,3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat CaVγ6. Anti-CACNG6 (extracellular) Antibody (#ACC-112) can be used in western blot and immunohistochemistry. The antibody recognizes an extracellular epitope and is thus ideal for detecting CaVγ6 in living cells. It has been designed to recognize CaVγ6 from rat, mouse and human samples.

For research purposes only, not for human use
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