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Anti-CACNG7 (extracellular) Antibody

CaVγ7, Voltage-dependent calcium channel gamma-7 subunit, TARP gamma-7, Transmembrane AMPAR regulatory protein gamma-7

Cat #: ACC-109
Alternative Name CaVγ7, Voltage-dependent calcium channel gamma-7 subunit, TARP gamma-7, Transmembrane AMPAR regulatory protein gamma-7
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)REKGR(S)VASEYFLE, corresponding to amino acid residues 67-80 of human CACNG7 (Accession P62955). 1st extracellular loop.
Accession (Uniprot) Number P62955
Gene ID 59284
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, mouse –13/14 amino acid residues identical.
RRID AB_2756548.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.85 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ifc*, ih*, ip*, lci*
Western blot
  • Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
    1,2. Anti-CACNG7 (extracellular) Antibody (#ACC-109), (1:1000).
    3,4. Anti-CACNG7 (extracellular) Antibody, preincubated with CACNG7 (extracellular) Blocking Peptide (#BLP-CC109).
References
  1. Cho, C.H. et al. (2007) Neuron. 55, 890.
  2. Hamad, M.I. et al. (2014) Development. 141, 1737.
  3. Studniarczyk, D. et al. (2013) Nat. Neurosci. 16, 1266.
Scientific background

Transmembrane AMPA receptor regulatory proteins (TARPs) serve as auxiliary subunits of AMPA receptors and regulate functional aspects of these receptors such as: fast excitatory synaptic transmission, surface trafficking, synaptic clustering and glutamate affinity. Generally, TARPs are responsible for regulating expression, channel properties and localization of AMPA receptors in the brain1.

TARPs are non-pore-forming integral membrane proteins with four transmembrane domains that are widely expressed in the CNS. TARP family is divided in two: Type I and type II. Type I TARPs include two calcium channel γ subunits: γ5 and γ7, also known as Cacng5 and Cacng7, respectively.They do not traffic AMPAR, they only modulate existing AMPAR channel function2.

CaVγ7 (Transmembrane AMPA Receptor Regulatory Protein Isoform γ7) is highly expressed in Purkinje cells of the cerebellum and in glomerular synapses in the granule cell layer of the cerebellum and significantly enhances glutamate-evoked currents and modulates gating of AMPARs. CaVγ7 appears to derive from cerebellar Golgi cells2,3.                       

CaVγ7 shows the largest sequence divergence compared to other TARP members. Its structure demonstrates a short C-terminus with an absence of the TTPV motif that is critical in the binding of TARP-associated AMPARs to PSD-95 and in clustering at the synapse3.

Several recent studies have suggested that CaVγ7 has the ability to associate with extrasynaptic calcium-permeable AMPA receptors and plays an important role in regulating the presence of neuronal calcium-permeable AMPA receptors at synapses3. In addition, transfection of TARP CaVγ7 causes AMPA receptors to resensitize upon continued glutamate application3.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 24/01/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human CaVγ7. Anti-CACNG7 Antibody (#ACC-109) can be used in western blot analysis. The antibody recognizes an extracellular epitope and is thus ideal for detecting CaVγ7 in living cells. It has been designed to recognize CaVγ7 from rat, mouse and human samples.

For research purposes only, not for human use
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