Name: Anti-CaVpan α1 Antibody
Brand: Alomone Labs
SKU: ACC-004

Overview

Cat #: ACC-004

Alternative Name Voltage-dependent L-type calcium channel subunit alpha-1C, Cacna1c

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)DNFDYLTRDWSILGPHHLD, corresponding to amino acid residues 1506-1524 of rat Ca_V1.2 (Accession P22002). Intracellular, C-terminus region.

Accession (Uniprot) Number P22002

Gene ID 24239

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Ca_V1 family (α_1C, α_1D, α_1F, α_1S) from all known species - identical; Ca_V2 family (α_1A, α_1B, α_1E) from all known species - 17/19 amino acid residues identical.

RRID AB_2039762.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN₃.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

Antibody concentration after reconstitution 0.3 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: if, ih, ip, wb

May also work in: ic*, ifc*

Western blot

Western blot analysis of rat brain membranes:
1. Anti-Ca_Vpan α1 Antibody (#ACC-004), (1:200).
2. Anti-Ca_Vpan α1 Antibody, preincubated with Pan CaV α 1 Blocking Peptide (#BLP-CC004).

Immunoprecipitation

Rat basilar artery lysates (Murphy, K. et al. (2003) Mol. Pharmacol. 64, 640.).

Immunohistochemistry

Human placenta (1:300) (Bernucci, L. et al. (2006) Placenta 27, 1082.).

References

Bonilla, M. and Cunningham, K.M. (2002) Sci. STKE 127, PE17.
Wu, X. et al. (2004) J. Biol. Chem. 279, 43392.
Iwasaki, S. et al. (2000) J. Neurosci. 20, 59.
Mark, M.D. and Herlitze, S. (2000) Eur. J. Biochem. 267, 5830.

Scientific background

Ca²⁺ ions regulate many cellular processes such as secretion, proliferation, and apoptosis and they are also cofactors of many proteins.^1,2 In neurons, Ca²⁺ entry through voltage-dependent Ca²⁺ (Ca_V) channels triggers neurotransmitter release. Fast synaptic transmission in the mammalian central nervous system (CNS) is mediated by several high voltage-dependent Ca²⁺ channels.³

Five types of Ca²⁺channels are expressed in the CNS: the L-type (Ca_V1), N-type (Ca_V2.2), P/Q-type (Ca_V2.1), R-type (Ca_V2.3), and the T-type (Ca_V3).⁴ Each Ca_Vchannel is a multimeric protein composed of a pore forming α1 subunit and the auxiliary β (Ca_Vβ), α2δ and γ subunits. There are four known Ca_Vβ subunits, in addition to four α2δ subunits and eight γ subunits.⁴

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Alomone Labs is pleased to offer an antibody directed against an epitope of rat Ca_V1.2 channel that is identical in all isoforms of Ca_V1 and Ca_V2. Anti-Ca_Vpan α1 Antibody (#ACC-004) can be used in western blot, immunoprecipitation and immunohistochemistry applications. It has been designed to recognize the high voltage-gated calcium channels, Ca_V1 and Ca_V2 subfamily members, from human, rat and mouse samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 03/01/2024

Applications

Citations

Western blot citations

Mouse eye lysate.
Grabner, C.P. et al. (2015) J. Neurosci. 35, 13133.
Mouse heart lysates (1:400).
Viola, H.M. et al. (2014) Proc. Natl. Acad. Sci. U.S.A. 111, E2905.

Immunoprecipitation citations

Mouse heart lysates (1.2 µg).
Viola, H.M. et al. (2014) Proc. Natl. Acad. Sci. U.S.A. 111, E2905.
Rat basilar artery lysates.
Murphy, K. et al. (2003) Mol. Pharmacol. 64, 640.

Immunohistochemistry citations

Mouse eye sections.
Grabner, C.P. et al. (2015) J. Neurosci. 35, 13133.
Crab cardiac ganglion (1:100).
Ransdell, J.L. et al. (2013) J. Neurophysiol. 110, 42.
Human placenta (1:300).
Bernucci, L. et al. (2006) Placenta 27, 1082.

More product citations

Fadool, D.A. et. al. (2004) Neuron 41, 389.
MacPherson, M.R. et. al. (2001) Am. J. Physiol. 280, C394.
Clarke, M.S. et al. (2000) J. Appl. Physiol 89, 731.
Pereon, Y. et al. (1998) Pflugers Arch. 436, 309.

Specifications

Scientific Background

Specific Control Product

Pan CaV α 1 Blocking Peptide (#BLP-CC004) is the original antigen used for immunization during Anti-Ca_Vpan α1 Antibody (#ACC-004) generation. The blocking peptide binds and ‘blocks’ Anti-Pan CaV α 1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control.

Pan CaV α 1 Blocking Peptide (#BLP-CC004)

Shipping and Ordering Information

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Anti-Ca_Vpan α1 Antibody