- Peptide (C)DNFDYLTRDWSILGPHHLD, corresponding to amino acid residues 1506-1524 of rat CaV1.2 (Accession P22002). Intracellular, C-terminus region.
- Western blot analysis of rat brain membranes:1. Anti-CaVpan α1 Antibody (#ACC-004), (1:200).
2. Anti-CaVpan α1 Antibody, preincubated with Pan CaV α 1 Blocking Peptide (#BLP-CC004).
- Rat basilar artery lysates (Murphy, K. et al. (2003) Mol. Pharmacol. 64, 640.).
- Human placenta (1:300) (Bernucci, L. et al. (2006) Placenta 27, 1082.).
Ca2+ ions regulate many cellular processes such as secretion, proliferation, and apoptosis and they are also cofactors of many proteins.1,2 In neurons, Ca2+ entry through voltage-dependent Ca2+ (CaV) channels triggers neurotransmitter release. Fast synaptic transmission in the mammalian central nervous system (CNS) is mediated by several high voltage-dependent Ca2+ channels.3
Five types of Ca2+ channels are expressed in the CNS: the L-type (CaV1), N-type (CaV2.2), P/Q-type (CaV2.1), R-type (CaV2.3), and the T-type (CaV3).4 Each CaV channel is a multimeric protein composed of a pore forming α1 subunit and the auxiliary β (CaVβ), α2δ and γ subunits. There are four known CaVβ subunits, in addition to four α2δ subunits and eight γ subunits.4
Species reactivity key:
Alomone Labs is pleased to offer an antibody directed against an epitope of rat CaV1.2 channel that is identical in all isoforms of CaV1 and CaV2. Anti-CaVpan α1 Antibody (#ACC-004) can be used in western blot, immunoprecipitation and immunohistochemistry applications. It has been designed to recognize the high voltage-gated calcium channels, CaV1 and CaV2 subfamily members, from human, rat and mouse samples.