Anti-CD39 (extracellular) Antibody

Ectonucleoside triphosphate diphosphohydrolase 1, NTPDase 1, Ecto-ATP diphosphohydrolase 1, Ecto-ATPDase 1, Ecto-apyrase, Lymphoid cell activation antigen, ENTPD1
  • New
Cat #: ANT-065
Alternative Name Ectonucleoside triphosphate diphosphohydrolase 1, NTPDase 1, Ecto-ATP diphosphohydrolase 1, Ecto-ATPDase 1, Ecto-apyrase, Lymphoid cell activation antigen, ENTPD1
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m
    May also work in: r*
      • Peptide (C)KTSYAGVKEKYLSE, corresponding to amino acid residues 398-411 of human CD39 (Accession P49961). Extracellular loop.
        Anti-CD39 (extracellular) Antibody
    Accession (Uniprot) Number P49961
    Gene ID 953
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse – 12/14 amino acid residues identical; rat – 11/14 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ifc, wb
    May also work in: ic*, ih*, ip*, lci*
      • Anti-CD39 (extracellular) Antibody
        Western blot analysis of human Jurkat T-cell leukemia cell line (lanes 1 and 3) and human THP-1 monocytic leukemia cell line (lanes 2 and 4) lysates:
        1,2. Anti-CD39 (extracellular) Antibody (#ANT-065), (1:400).
        3,4. Anti-CD39 (extracellular) Antibody, preincubated with the negative control antigen.
        Anti-CD39 (extracellular) Antibody
        Western blot analysis of mouse BV-2 microglia cell line (lanes 1 and 3) and human HMC3 microglia cell line (lanes 2 and 4) lysates:
        1,2. Anti-CD39 (extracellular) Antibody (#ANT-065), (1:200).
        3,4. Anti-CD39 (extracellular) Antibody, preincubated with the negative control antigen.
        Anti-CD39 (extracellular) Antibody
        Western blot analysis of mouse brain membranes:
        1. Anti-CD39 (extracellular) Antibody (#ANT-065), (1:200).
        2. Anti-CD39 (extracellular) Antibody, preincubated with the negative control antigen.
      • Anti-CD39 (extracellular) Antibody
        Cell surface detection of CD39 in live intact human THP-1 monocytic leukemia cells:
        ___ Cells.
        ___ Cells + goat anti-rabbit-FITC.
        ___ Cells + Anti-CD39 (extracellular) Antibody (#ANT-065), (2.5 µg) + goat anti-rabbit-FITC.
    References
      • Ectonucleoside triphosphate diphosphohydrolase 1 (NTPDase1, ENTPD1, or CD39) is an enzyme that catalyzes the phosphohydrolysis of extracellular adenosine triphosphate (eATP) and diphosphate (eADP), that are released following inflammatory stress and cell injury, to adenosine monophosphate (AMP). AMP is then used by the ecto-5’-nucleotidase CD73 to synthesize adenosine1.

        Hydrolysis of adenosine triphosphate and adenosine diphosphate, performed by CD39, suppresses the immune system and inhibits T-cell and Natural killer (NK) cell responses. This inhibition has a role in promoting Chronic lymphocytic leukemia (CLL) and hence it is proposed that blockade or inhibition of CD39 may be a target for new immune therapy for CLL2. CD39 activity is also related to the occurrence of calcific aortic valve disease (CAVD)3, and to various other types of immune responses4. In addition, CD39 plays a dominant role in the purinergic regulation of inflammation1.

        The transcription of CD39 is constitutive in microvascular endothelium and certain immune cells. The modulated expression of NTPDase1 has been closely associated with inflammatory cytokines, oxidative stress and hypoxia in vitro and in vivo5.

        Localization of CD39 within lipid rafts suggests that this ecto-enzyme may be involved in cell-cell contact and signaling6.

        CD39 is a part of the ectonucleoside triphosphate dyphosphohydrolases (eNTDPase) protein family which shares a unique structural feature: instead of being anchored in the membrane by a single transmembrane domain or lipid link like other ectoenzymes, these proteins contain two transmembrane domains, one at each end of the protein. The active site lies in the large extracellular region. In addition, all family members share a set of short sequences termed apyrase conserved regions (ACRs)7.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IF- Immunofluorescence, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/10/2019

    Anti-CD39 (extracellular) Antibody (#ANT-065) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize ENTPD1 from rat, mouse, and human samples.

    For research purposes only, not for human use