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Peptide (C)KGSTLKKAFEHSVHR, corresponding to amino acid residues 429-433 of mouse CD73 (Accession Q61503). Extracellular, N-terminus.
Western blot analysis of mouse brain membranes (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):1,2. Anti-CD73 (extracellular) Antibody (#ANT-066), (1:200).
3,4. Anti-CD73 (extracellular) Antibody, preincubated with the control peptide antigen.Western blot analysis of human U-87 MG glioblastoma cell line lysate (lanes 1 and 3) and human MDA-MB-231 breast adenocarcinoma cell line lysate (lanes 2 and 4):1,2. Anti-CD73 (extracellular) Antibody (#ANT-066), (1:200).
3,4. Anti-CD73 (extracellular) Antibody, preincubated with the control peptide antigen.
Expression of CD73 in mouse striatum and hippocampus.Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-CD73 (extracellular) Antibody (#ANT-066), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. CD73 staining (green) in mouse striatum is detected in striatal cells (arrows). B. CD73 staining (green) in hippocampal CA1 region is detected in cells of the pyramidal layer (vertical arrow) and their apical dendrites (horizontal arrows). Cell nuclei are stained with DAPI (blue).
Cell surface detection of CD73 in live intact human Jurkat T-cell leukemia cells:___ Cells.
___ Cells + goat-anti-rabbit-FITC.
___ Cells + Anti-CD73 (extracellular) Antibody (#ANT-066), (5 µg) + goat-anti-rabbit-FITC.
CD73 (ecto-5′-nucleotidase, NT5E) is a glycosylphosphatidylinositol enzyme expressed on the cell surface of various cell types. CD73 is responsible for hydrolyzing extracellular adenosine monophosphate to adenosine and inorganic phosphate, which results in activation of the G-protein coupled receptors, namely A2AR and A2BR, to exert potent immunoregulatory activity.
CD73 plays an important role in homeostatic functions in healthy organs and tissues. The protein is involved as an inhibitory immune checkpoint molecule, it promotes immune escape of tumor cells since free adenosine generated by the protein inhibits cellular immune responses1,2.
CD73 primarily detected in cancer cells and in immune cells such as CD4+Foxp3+ regulatory T cells, and myeloid-derived suppressor cells that are recruited by the tumor2.
Studies show that CD73 expression is significantly up-regulated in numerous malignant tumors such as breast cancer, colorectal cancer, prostate cancer, ovarian cancer, and gallbladder cancer3.
Species reactivity key:
Anti-CD73 (extracellular) Antibody (#ANT-066) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize NT5E from human, rat and mouse samples.