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Anti-CNTF Antibody

Ciliary neurotrophic factor
Cat #: ANT-031
Alternative Name Ciliary neurotrophic factor
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)ELSQWTVRSIHDLR, corresponding to amino acid residues 164-177 of human CNTF (Accession P26441). C-terminus.
Accession (Uniprot) Number P26441
Gene ID 1270
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, mouse - identical.
RRID AB_10917441.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS) pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ih, wb
May also work in: ifc*, ip*
Western blot
Immunohistochemistry
  • Expression of Ciliary neurotrophic factor (CNTF) in rat hippoccampus
    Expression of Ciliary neurotrophic factor (CNTF) in rat hippoccampus
    Immunohistochemical staining of rat hippoccampus using Anti-CNTF Antibody (#ANT-031), (1:200). A. CNTF staining (green) reveals astrocyte-profiles (arrows) in the hilus (H) of the dentate gyrus. B. Staining the same section with the astrocyte marker, anti-glial fibrillary acidic protein (red), visualizes astrocytes in the hilus. C. Merge of the two images shows that CNTF is indeed expressed in astrocytes.
Immunocytochemistry
  • Expression of CNTF in rat C6 glioma cells
    Expression of CNTF in rat C6 glioma cells
    Immunocytochemical staining of fixed and permeabilized rat C6 glioma cells. A. Cells were stained with Anti-CNTF Antibody (#ANT-031), (1:200), followed by goat anti-rabbit-DyLight-594 secondary antibody (red). B. Cell nuclei were visualized with the membrane-permeable DNA dye Hoechst 33342 (blue staining). C. Live view of the cells.
References
  1. Mufson, E.J. et al. (1999) Prog. Neurobiol. 57, 451.
  2. Adler, R. et al. (1979) Science 204, 1434.
  3. Murphy, M. et al. (1997) Prog. Neurobiol. 52, 355.
  4. Henderson, J.T. et al. (1994) Mol. Brain Res. 22, 151.
  5. Stahl, N. et al. (1993) J. Biol. Chem. 268, 7628.
  6. Richardson, P.M. (1994) Pharmacol. Ther. 63, 187.
  7. Ip, N.Y. et al. (1991) J. Physiol. 85, 123.
  8. Gurney, M.E. et al. (1992) J. Neurosci. 12, 3241.
  9. Sendtner, M. et al. (1992) Nature 358, 502.
  10. Mayer, M. et al. (1994) Development 120, 143.
Scientific background

CNTF is a polypeptide trophic factor, member of the alpha-helical cytokine superfamily1. It was initially purified from the chick eye on the basis of its ability to promote survival of E8 chick ciliary ganglion neurons in culture2. CNTF is synthesized by glia both in the CNS and PNS3 and it has been demonstrated that CNTF is ubiquitously distributed in neurons and glia throughout the rodent brain4. CNTF effects are mediated by a tripartite receptor complex consisting of two signal-transducing subunits (leukemia inhibitory factor receptor, gp130) and a CNTF-specific ligand-binding-subunit (CNTFR)5.

CNTF can support the survival of many different cell populations within the PNS and the CNS6. in vitro, CNTF promotes proliferation and neuronal specifications in hippocampal neurons. in vivo, it supports the viability of non-primate motor neurons7, induces sprouting of cholinergic motor neurons8 and delays neural degeneration in genetic models of motor neuron disease9. In addition, it is involved in the development stage of astrocytes and oligodendocytes10.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 21/12/2020

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human CNTF. Anti-CNTF Antibody (#ANT-031) can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize CNTF from human, rat and mouse samples. Anti-CNTF Antibody specifically recognizes CNTF and not related cytokines.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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