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Anti-CNTFRα (extracellular) Antibody

Ciliary neurotrophic factor receptor subunit alpha, CNTF receptor alpha, CNTFR alpha
Cat #: ACR-051
Alternative Name Ciliary neurotrophic factor receptor subunit alpha, CNTF receptor alpha, CNTFR alpha
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide CHIRYMHLFSTIKYK, corresponding to amino acid residues 164-178 of rat CNTFRα (Accession Q08406). Extracellular.
Accession (Uniprot) Number Q08406
Gene ID 313173
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human - identical.
RRID AB_2340925.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*, lci*
Western blot
  • Western blot analysis of rat brain (lanes 1 and 4), rat cortex (lanes 2 and 5) and mouse brain (lanes 3 and 6) lysates:
    Western blot analysis of rat brain (lanes 1 and 4), rat cortex (lanes 2 and 5) and mouse brain (lanes 3 and 6) lysates:
    1-3. Anti-CNTFRα (extracellular) Antibody (#ACR-051), (1:200).
    4-6. Anti-CNTFRα (extracellular) Antibody, preincubated with CNTFRα (extracellular) Blocking Peptide (#BLP-CR051).
Immunohistochemistry
  • Expression of CNTF receptor α in rat hippocampus
    Expression of CNTF receptor α in rat hippocampus
    Immunohistochemical staining of rat hippocampal CA1 region using Anti-CNTFRα (extracellular) Antibody (#ACR-051), (1:400). A. CNTFRα staining (green) appears in astrocytes in the CA1 region (arrows). B. Nuclei staining using DAPI as the counterstain (green). C. Merged image of panels A and B.
References
  1. Mufson, E.J. et al. (1999) Prog. Neurobiol. 57, 451.
  2. Adler, R. et al. (1979) Science 204, 1434.
  3. Murphy, M. et al. (1997) Prog. Neurobiol. 52, 355.
  4. Henderson, J.T. et al. (1994) Mol. Brain Res. 22, 151.
  5. Stahl, N. et al. (1993) J. Biol. Chem. 268, 7628.
  6. Davis, S. et al. (1993) Science 259, 1736.
  7. Vergara, C. and Ramirez, B. (2004) Brain Res. Rev. 47, 161.
  8. Lelievre, E. et al. (2001) J. Biol. Chem. 276, 22476.
Scientific background

CNTF is a polypeptide trophic factor, member of the alpha-helical cytokine superfamily1. It was initially purified from the chick eye on the basis of its ability to promote survival of E8 chick ciliary ganglion neurons in culture2. CNTF is synthesized by glia both in the CNS and PNS3 and it has been demonstrated that CNTF is ubiquitously distributed in neurons and glia throughout the rodent brain4. CNTF effects are mediated by a tripartite receptor complex consisting of two signal-transducing subunits (leukemia inhibitory factor receptor, gp130) and a CNTF-specific ligand-binding-subunit (CNTFRα)5.

CNTFRα is anchored to the membrane by a glycosyl-phosphatidylinositol (GPI) linkage and lacks the intracellular signaling moiety. It can be cleaved from the GPI anchor to yield a soluble form of the receptor (sCNTFRα)6,7.

CNTF binds to CNTFRα, the latter associates with gp130 which recruits LIFR. This leads to the activation of a signaling cascade involving the JAK/STAT pathway7.

CNTFRα knockout mice die within 24 hours after birth as opposed to CNTF knock animals which are viable. This strongly suggest that CNTFRα has other ligands besides CNTF. Indeed, CLC/CLF complex (cardiotrophin like cytokine/cytokine-like factor-1) binds CNTFRα and activates a similar signaling pathway to that of CNTF8.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 21/12/2020

Anti-CNTFRα (extracellular) Antibody (#ACR-051) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and can potentially detect the receptor in living cells. It has been designed to recognize CNTF receptor α from human, mouse, and rat samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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