Anti-Connexin-26 (GJB2) Antibody

CX26, Gap junction β2 protein
    Cat #: ACC-212
    Alternative Name CX26, Gap junction β2 protein
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
      • Peptide (C)HEKKRKFMKGEIK, corresponding to amino acid residues 100-112 of rat Connexin-26 (Accession P21994). Intracellular, cytoplasmic loop.
        Anti-Connexin-26 (GJB2) Antibody
    Accession (Uniprot) Number P21994
    Gene ID 394266
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - identical; human - 12/13 amino acid residues identical.
    RRID AB_11124274.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ih, wb
    May also work in: ifc*, ip*
      • Anti-Connexin-26 (GJB2) Antibody
        Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) liver membranes:
        1,2. Anti-Connexin-26 (GJB2) Antibody (#ACC-212), (1:200).
        3,4. Anti-Connexin-26 (GJB2) Antibody, preincubated with the negative control antigen.
      • Human fetal cochlear sections (Locher, H. et al. (2015) Dev. Neurobiol. 75, 1219.).
      • Anti-Connexin-26 (GJB2) Antibody
        Expression of Connexin-26 in human HepG2 liver hepatocellular carcinoma cells
        Immunocytochemical staining of fixed and permeabilized HepG2 liver hepatocellular carcinoma cells. A. Cells were stained with Anti-Connexin-26 (GJB2) Antibody (#ACC-212), (1:400), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Cell nuclei were visualized using Hoechst 33342 (blue). C. Merge of the two images.
    References
      • Connexins (Cx) are integral membrane proteins consisting of four transmembrane domains, two extracellular loops, one intracellular loop and intracellular N- and C-termini. The 21 members belonging to this family form homomeric or heteromeric hexamers generally termed connexons or hemi-channels. In turn, these hemi-channels further assemble in a head-to-head manner, thus forming gap junction channels1,2. Connexins are ubiquitously expressed and their activity is regulated at the expression level and by post-translational modifications1.

        Gap junctions are usually found in clusters and enable intercellular communication by allowing the passage of small molecules between cells3. They play important roles in different biological processes. These include differentiation, cell cycle synchronization, cellular development, neuronal activity and the immune response2,4,5.

        Due to their important roles, mutations in connexins are linked with a number of diseases such as neurodegenerative disorders, skin diseases and developmental abnormalities2,5,6. Mutations in the gene encoding for Connexin-26 protein are associated with deafness7.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Anti-Connexin-26 (GJB2) Antibody (#ACC-212) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize Cx26 from human, rat, and mouse samples.

    For research purposes only, not for human use