- Peptide (C)HEKKRKFMKGEIK, corresponding to amino acid residues 100-112 of rat Connexin-26 (Accession P21994). Intracellular, cytoplasmic loop.
- Rat and mouse liver membranes (1:200).
- Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) liver membranes:1,2. Anti-Connexin-26 (GJB2) Antibody (#ACC-212), (1:200).
3,4. Anti-Connexin-26 (GJB2) Antibody, preincubated with Connexin-26/GJB2 Blocking Peptide (#BLP-CC212).
- Human fetal cochlear sections (Locher, H. et al. (2015) Dev. Neurobiol. 75, 1219.).
- Fixed and permeabilized human HepG2 liver hepatocellular carcinoma cells (1:400).
Connexins (Cx) are integral membrane proteins consisting of four transmembrane domains, two extracellular loops, one intracellular loop and intracellular N- and C-termini. The 21 members belonging to this family form homomeric or heteromeric hexamers generally termed connexons or hemi-channels. In turn, these hemi-channels further assemble in a head-to-head manner, thus forming gap junction channels1,2. Connexins are ubiquitously expressed and their activity is regulated at the expression level and by post-translational modifications1.
Gap junctions are usually found in clusters and enable intercellular communication by allowing the passage of small molecules between cells3. They play important roles in different biological processes. These include differentiation, cell cycle synchronization, cellular development, neuronal activity and the immune response2,4,5.
Due to their important roles, mutations in connexins are linked with a number of diseases such as neurodegenerative disorders, skin diseases and developmental abnormalities2,5,6. Mutations in the gene encoding for Connexin-26 protein are associated with deafness7.