Anti-Connexin-29 (extracellular) Antibody

CX29, Gap junction gamma-3 protein, Gap junction epsilon-1 protein, GJC3, GJE1
  • New
Cat #: ACC-223
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: m, r
    Immunogen
    Peptide (C)GNDENEFI(S)HLGQPG, corresponding to amino acid residues 56-70 of mouse Connexin-29 (Accession Q921C1). 1st extracellular loop.
    Accession (Uniprot) Number Q921C1
    Gene ID 118446
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse – 14/15 amino acid residues identical; rat – 13/15 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Specificity Will not recognize human samples.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic, ifc, ih, ip, lci
    Western blot
    Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates:
    1,2. Anti-Connexin-29 (extracellular) Antibody (#ACC-223), (1:200).
    3,4. Anti-Connexin-29 (extracellular) Antibody, preincubated with the control peptide antigen.
    References
    1. Nishii, K. et al. (2014) World J. Stem Cells 6, 571.
    2. Shimaoka, M. et al. (2019) Cancers (Basel) 11, 1.
    3. Wong, S.H. et al. (2017) Int. J. Med. Sci. 14, 246.
    4. Moon, Y. et al. (2010) Neuroreport 21, 1135.
    Scientific background

    Gap junction channels play an important role in intercellular communication, important for maintaining cell homeostasis. The connexin (Cx) gene family encodes hexameric hemichannels called connexons. When those connexons are docked head to head they form channels. Their presence between adjacent cells form gap junctions. One gap junction is composed of two hexameric connexons and 12 connexins form a single channel1. Connexins have four transmembrane domains, two extracellular loops and one cytoplasmic loop and cytosolic amino and carboxyl termini2.

    Connexins are responsible for transporting ions and small molecules between the cytoplasm of two attaching cells and between the cytoplasm and the extracellular space2.

    21 connexins have been identified in humans and 20 in mice2. Connexin 29 was shown to be expressed in the cochlear tissue of mice and rats and in the oligodendrocytes and myelinating Schwann cells in the normal cerebral cortex3,4.

    Studies show that in mice, connexin 29 expression increases in the penumbra region following traumatic brain injury. Increased expression is also observed in the astrocytes and microglia localized in the injury border4.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat

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    Last update: 14/03/2019

    Anti-Connexin-29 (extracellular) Antibody (#ACC-223) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize Connexin-29 from rat and mouse samples. Will not recognize human samples.

    For research purposes only, not for human use