Anti-Connexin-31 (GJB3) Antibody

CX31, Gap junction β3 protein
    Cat #: ACC-213
    Alternative Name CX31, Gap junction β3 protein
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: m, r
      • Peptide (C)EINKLLSEQDGSLK, corresponding to amino acid residues 216-228 of mouse Connexin-31 (Accession P28231). Intracellular, C-terminus
        Anti-Connexin-31 (GJB3) Antibody
    Accession (Uniprot) Number P28231
    Gene ID 14620
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat – 11/13 amino acid residues identical.
    RRID AB_2756554.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Specificity Not recommended for human samples.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: wb
    May also work in: ic*, ifc*, ih*, ip*
      • Anti-Connexin-31 (GJB3) Antibody
        Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes:
        1,2. Anti-Connexin-31 (GJB3) Antibody (#ACC-213), (1:200).
        3,4. Anti-Connexin-31 (GJB3) Antibody, preincubated with the negative control antigen.
    References
    • 1. He, L.Q. et al. (2005) Acta Biochim. Biophys. Sin. (Shanghai) 37, 547.
    • 2. Terrinoni, A. et al. (2004) J. Invest. Dermatol. 122, 837.
    • 3. Tattersall, D. et al. (2009) Hum. Mol. Genet. 18, 4734.
    • 4. Chi, J. et al. (2012) PLoS One 7, e32531.
    • 5. Abrams, C.K. et al. (2006) Proc. Natl. Acad. Sci. U.S.A. 103, 5213.
      • Connexins (Cxs) are the major components of gap junctions, aqueous pores that connect the cytoplasms of two adjacent cells and allow the transfer of ions and small molecules less than 1 kDa between them. Connexins structure includes four trans-membrane domains M1–M4, two extracellular domains E1–E2 and three cytoplasmic domains. The cytoplasmic domains include cytoplasmic loop and carboxy-terminus which are characteristic of each connexin. The membrane spanning and the extracellular domain are highly conserved1,2.

        Six connexins proteins forms a connexon, also called hemichannel, which can traffic to the plasma membrane and dock with a hemichannel from an adjacent cell to form an intercellular channel.  Paracrine signaling and the propagation of Ca2+ waves are two examples of hemichannels functional roles at the plasma membrane. To date, 19 connexin genes have been found in the mouse genome and 20 in the human genome1,3.

        Connexin 31 (CX31) is highly expressed in the stratum granulosum in the upper differentiating layer of the epidermis. Expression is also detected in peripheral nerves and the cochlea, testis of adults, in placenta, and in developing hindbrain4,5.

        Mutations in Connexin 31 are associated with multiple human diseases including the genetic skin disorder erythrokeratodermia variabilis, hearing impairment and peripheral neuropathy4.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Anti-Connexin-31 (GJB3) Antibody (#ACC-213) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize Cx31 from rat and mouse samples. It will unlikely recognize the protein from human samples.

    For research purposes only, not for human use