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Anti-Connexin-36 Antibody

Gap junction α9 protein, CX36, GJA9, GJD2
Cat #: ACC-209
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
    • Type: Polyclonal
    Source: Rabbit
    Reactivity: m, r
    May also work in: h
    Immunogen
    Peptide (C)EVKELAPHPSGLR, corresponding to amino acid residues 170-182 of rat Connexin-36 (Accession O70610). Intracellular loop.
    Accession (Uniprot) Number O70610
    Gene ID 50564
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, human - 12/13 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20ºC.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ih, wb
    May also work in: ic, ifc, ip
    Western blot
    Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes: 
    1, 2. Anti-Connexin-36 Antibody (#ACC-209), (1:200).
    3, 4. Anti-Connexin-36 Antibody, preincubated with the control peptide antigen.
    Immunohistochemistry
    Expression of Connexin-36 in rat spinal cord
    Immunohistochemical staining of perfusion-fixed frozen rat spinal cord sections using Anti-Connexin-36 Antibody (#ACC-209), (1:300). Connexin-36 staining (in red) appears in processes (arrows) along the first layer of the dorsal horn of the rat spinal cord. Cell nuclei are stained with DAPI (blue).
    References
    1. Peng, Y.F. et al. (2012) Chin. Med. J. 125, 2365.
    2. Condorelli, D.F. et al. (2000) Brain Res. Brain. Res. Rev. 32, 72.
    3. Condorelli, D.F. et al. (2003) Eur. J. Neurosci. 18, 1807.
    4. Marina, N. et al. (2008) Auton. Neurosci. 139, 15.
    5. Hartfield, E.M. et al. (2011) PLoS ONE 6, e14746.
    6. Wang, Y. et al. (2012) J. Neurosci. 32, 713.
    Scientific background

    The structural unit of the gap junctions is the connexon. Connexons are hexameric structures, formed by transcellular channels composed of connexins. Connexins (Cx) share a common structural basis, which is a four-transmembrane spanning protein, a cytoplasmic loop, two extracellular loops (E1–E2), one intracellular amino-(NT domain) and a carboxy-terminal (CT domain) region1.

    Connexin 36 (Cx36) is predominantly expressed in mammalian neuronal cells2. Cx36 is expressed in a variety of mammalian brain regions, but expression is particularly notable in the inferior olive, cerebellar cortex, olfactory bulbs, several brainstem nuclei, the CA3 subregion of the hippocampus and reticular thalamic nuclei3. Outside of the central nervous system, Cx36 also appears in motoneurons of adult rat spinal cord 4.

    Studies have shown that Cx36 is involved in some neural functions, including neuronal neurogenesis, neural fertility control and regulation of waking and sleep states, and also Cx36 plays an important role in N-methyl-D-aspartate-mediated neuronal death following neuronal injury5. A number of studies performed in humans and animals have confirmed the role of Cx36 in the generation and maintenance of seizure activity6.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Lyophilized PowderControl Antigen Included
    receive a 25 µl free trial sample!
    Image & Title: Anti-Connexin-36 Antibody
    Expression of Cx36 in rat brainstem.Immunohistochemical staining of rat brainstem sections using Anti-Connexin-36 Antibody (#ACC-209). Cx36 staining (red) is localized to hypoglossal motoneurons, as is SMI 32, a motoneuron marker (green). DAPI is used to stain nuclei.Adapted from Corsini, S. et al. (2017) Cell Death Dis. 8, e2881. with permission of Nature Publishing Group.
    Last update: 11/03/2019

    Anti-Connexin-36 Antibody (#ACC-209) is a highly specific antibody targeted against an epiotpe of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Connexin-36 from human, rat, and mouse samples.

    For research purposes only, not for human use
    Citations
    Immunohistochemistry citations
    1. Rat brainstem sections.
      Corsini, S. et al. (2017) Cell Death Dis. 8, e2881.
    Related Products
      • Antibodies for immuno-colocalization experiments
        1. Anti-Connexin-36-ATTO-488 Antibody (#ACC-209-AG).
          A fluorescent labeled primary antibody. It can be used in immuno-colocalization studies in conjunction with any of our antibodies raised in rabbit.