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Anti-Connexin-36 Antibody

CX36, Gap junction δ2 protein, Gap junction α9 protein, GJD2, GJA9

Cat #: ACC-209
Alternative Name CX36, Gap junction δ2 protein, Gap junction α9 protein, GJD2, GJA9
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: m, r
May also work in: h*
Immunogen
  • Peptide (C)EVKELAPHPSGLR, corresponding to amino acid residues 170-182 of rat Connexin-36 (Accession O70610). Intracellular loop.
Accession (Uniprot) Number O70610
Gene ID 50564
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse, human - 12/13 amino acid residues identical.
RRID AB_2340893.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*
Western blot
  • Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes: 
    Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain membranes: 
    1,2. Anti-Connexin-36 Antibody (#ACC-209), (1:200).
    3,4. Anti-Connexin-36 Antibody, preincubated with Connexin-36 Blocking Peptide (#BLP-CC209).
Immunohistochemistry
  • Expression of Connexin-36 in rat spinal cord
    Expression of Connexin-36 in rat spinal cord
    Immunohistochemical staining of perfusion-fixed frozen rat spinal cord sections using Anti-Connexin-36 Antibody (#ACC-209), (1:300). Cx36 staining (in red) appears in processes (arrows) along the first layer of the dorsal horn of the rat spinal cord. Cell nuclei are stained with DAPI (blue).
References
  1. Peng, Y.F. et al. (2012) Chin. Med. J. 125, 2365.
  2. Condorelli, D.F. et al. (2000) Brain Res. Brain. Res. Rev. 32, 72.
  3. Condorelli, D.F. et al. (2003) Eur. J. Neurosci. 18, 1807.
  4. Marina, N. et al. (2008) Auton. Neurosci. 139, 15.
  5. Hartfield, E.M. et al. (2011) PLoS ONE 6, e14746.
  6. Wang, Y. et al. (2012) J. Neurosci. 32, 713.
Scientific background

The structural unit of the gap junctions is the connexon. Connexons are hexameric structures, formed by transcellular channels composed of connexins. Connexins (Cx) share a common structural basis, which is a four-transmembrane spanning protein, a cytoplasmic loop, two extracellular loops (E1–E2), one intracellular amino-(NT domain) and a carboxy-terminal (CT domain) region1.

Connexin 36 (Cx36) is predominantly expressed in mammalian neuronal cells2. Cx36 is expressed in a variety of mammalian brain regions, but expression is particularly notable in the inferior olive, cerebellar cortex, olfactory bulbs, several brainstem nuclei, the CA3 subregion of the hippocampus and reticular thalamic nuclei3. Outside of the central nervous system, Cx36 also appears in motoneurons of adult rat spinal cord 4.

Studies have shown that Cx36 is involved in some neural functions, including neuronal neurogenesis, neural fertility control and regulation of waking and sleep states, and also Cx36 plays an important role in N-methyl-D-aspartate-mediated neuronal death following neuronal injury5. A number of studies performed in humans and animals have confirmed the role of Cx36 in the generation and maintenance of seizure activity6.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Lyophilized Powder
Image & Title: Anti-Connexin-36 Antibody
Expression of Cx36 in rat brainstem.Immunohistochemical staining of rat brainstem sections using Anti-Connexin-36 Antibody (#ACC-209). Cx36 staining (red) is localized to hypoglossal motoneurons, as is SMI 32, a motoneuron marker (green). DAPI is used to stain nuclei.Adapted from Corsini, S. et al. (2017) Cell Death Dis. 8, e2881. with permission of Nature Publishing Group.
Last update: 25/07/2021

Anti-Connexin-36 Antibody (#ACC-209) is a highly specific antibody targeted against an epiotpe of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Cx36 from human, rat, and mouse samples.

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For research purposes only, not for human use

Applications

Citations

Citations
Immunohistochemistry citations
  1. Rat brainstem sections.
    Corsini, S. et al. (2017) Cell Death Dis. 8, e2881.

Specifications

Scientific Background

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Shipping and Ordering information

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