- Peptide (C)HRLARASEQERR, corresponding to amino acid residues 100 - 111 of rat Connexin-47 (Accession Q80XF7). Intracellular loop.
- Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4):1-2. Anti-Connexin-47 (GJC2) Antibody (#ACC-222), (1:200).
3-4. Anti-Connexin-47 (GJC2) Antibody, preincubated with Connexin-47 (GJC2) Blocking Peptide (BLP-CC222).
- Western blot analysis of human U-87 MG glioblastoma cell line lysate:1. Anti-Connexin-47 (GJC2) Antibody (#ACC-222), (1:200).
2. Anti-Connexin-47 (GJC2) Antibody, preincubated with Connexin-47 (GJC2) Blocking Peptide (BLP-CC222).
Connexin-47 (Cx47) is a gap junction (GJ) protein expressed in the Central nervous system.
It belongs to the connexins family of transmembrane proteins. Proteins that belong to this family form intercellular membrane channels that allow diffusional movement of ions, metabolites, and signaling molecules. The family members are composed of four transmembrane segments, an unstructured C and N cytoplasmic termini, a cytoplasmic loop and two extra-cellular loops1.
Connexins oligomerize among the family members to form different structures and Cx47 was shown to form gap junction channels together with Cx43.
These voltage gated channels exhibit ionic and chemical rectification, which creates a directional diffusion barrier for the movement of ions and negatively charged molecules from cells expressing Cx47 to those with Cx431.
The channel mediates an intercellular communication between oligodendrocytes and between astrocytes. This process is essential to maintain the integrity of myelin and maintaining K+ buffering and nutrient homeostasis in oligodendrocytes, myelin and oligodendrocyte function2,3, and indeed, mutations in human Cx47 cause severe myelin disorders2.
Cx43/Cx47 gap junction channels activity was shown to be associated with the development of Multiple Sclerosis and autoimmune demyelination, underscoring a critical role for Cx47 in regulating neuroinflammation4.