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Anti-Cortactin Antibody

Src substrate cortactin, Oncogene EMS1, EMS1, Amplaxin, CTTN
Cat #: APZ-018
Alternative Name Src substrate cortactin, Oncogene EMS1, EMS1, Amplaxin, CTTN
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)KTSNIRANFENLAK, corresponding to amino acid residues 346 - 359 of mouse Cortactin (Accession Q60598). Intracellular.
Accession (Uniprot) Number Q60598
Gene ID 13043
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, human – identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: wb
May also work in: ic*, ih*, ip*
Western blot
  • Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
    Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):
    1, 2. Anti-Cortactin Antibody (#APZ-018), (1:1000).
    3, 4. Anti-Cortactin Antibody, preincubated with Cortactin Blocking Peptide (#BLP-PZ018).
  • Western blot analysis of human K562 erythroleukemia cell line lysate (lanes 1 and 5), human HeLa cervical adenocarcinoma cell line lysate (lanes 2 and 6), human U-87MG glioblastoma cell line lysate (lanes 3 and 7) and human MCF-7 breast adenocarcinoma cell line lysate (lanes 4 and 8):
    Western blot analysis of human K562 erythroleukemia cell line lysate (lanes 1 and 5), human HeLa cervical adenocarcinoma cell line lysate (lanes 2 and 6), human U-87MG glioblastoma cell line lysate (lanes 3 and 7) and human MCF-7 breast adenocarcinoma cell line lysate (lanes 4 and 8):
    1-4. Anti-Cortactin Antibody (#APZ-018), (1:200).
    5-8. Anti-Cortactin Antibody, preincubated with Cortactin Blocking Peptide (#BLP-PZ018).
References
  1. Yin, M. et al. (2017) Oncotarget 8, 88232.
  2. Shvetsov, A. et al. (2009) Cell Motil. Cytoskeleton 66, 90.
  3. Scherer, A.N. et al. (2018) J. Biol. Chem. 293, 13022.
Scientific background

Cortical acting binding protein, Cortactin, is an actin-binding protein involved in cytoskeletal regulation1. Cortactin is a 63–65 kDa protein that consists of four major domains: N-terminal acidic (NTA), 6.5 tandems repeat, proline-rich domain, and the C-terminal Src homology 3 domains (SH3 domains)2 and it is regulated by post translational modifications such as phosphorylation and acetylation1.

The actin filament network provides mechanical support as well as protrusive and contractile forces essential for directed cell motility and cell and tissue morphogenesis. Cortactin activates the Arp2/3 complex, the main regulator of actin polymerization, and thereby regulates the assembly of branched actin3.

Overexpression of cortactin was shown to enhance cell migration, invasion, and tumor cell metastasis. It is also overexpressed in various types of cancer such as breast cancers as well as head and neck tumors1.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

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Last update: 20/10/2020

Anti-Cortactin Antibody (#APZ-018) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize Cortactin from rat, mouse, and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
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