Free shipping starts now, no minimum, no coupons required!

Anti-CRF1/CRHR1 (extracellular) Antibody

Corticotropin-releasing factor receptor 1, CRFR1, Corticotropin-releasing hormone receptor 1, CRH-R1, CRH1
Cat #: ACR-050
Alternative Name Corticotropin-releasing factor receptor 1, CRFR1, Corticotropin-releasing hormone receptor 1, CRH-R1, CRH1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide ENLSLTSNVSGLQC, corresponding to amino acid residues 31-44 of rat CRHR1 (Accession P35353). Extracellular, N-terminus.
Accession (Uniprot) Number P35353
Gene ID 58959
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse –12/14 amino acid residues identical; human – 11/14 amino acid residues identical.
RRID AB_2756576.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.85 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: if, ih, wb
May also work in: ic*, ifc*, ip*, lci*
Western blot
  • Western blot analysis of mouse brain membrane (lanes 1 and 4), rat brain membrane (lane 2 and 5) and rat cortex lysate (lane 3 and 6):
    Western blot analysis of mouse brain membrane (lanes 1 and 4), rat brain membrane (lane 2 and 5) and rat cortex lysate (lane 3 and 6):
    1-3. Anti-CRF1/CRHR1 (extracellular) Antibody (#ACR-050), (1:200).
    4-6. Anti-CRF1/CRHR1 (extracellular) Antibody, preincubated with CRF1/CRHR1 (extracellular) Blocking Peptide (#BLP-CR050).
  • Western blot analysis of human neuroblastoma (SH-SY5Y) (lanes 1 and 4), human brain astrocytoma (CCF-STTGI) (lanes 2 and 5) and rat pheochromocytoma (PC12) (lanes 3 and 6):
    Western blot analysis of human neuroblastoma (SH-SY5Y) (lanes 1 and 4), human brain astrocytoma (CCF-STTGI) (lanes 2 and 5) and rat pheochromocytoma (PC12) (lanes 3 and 6):
    1-3. Anti-CRF1/CRHR1 (extracellular) Antibody (#ACR-050), (1:200).
    4-6. Anti-CRF1/CRHR1 (extracellular) Antibody, preincubated with CRF1/CRHR1 (extracellular) Blocking Peptide (#BLP-CR050).
Immunohistochemistry
  • Expression of Corticotropin-releasing factor receptor 1 in mouse brain
    Expression of Corticotropin-releasing factor receptor 1 in mouse brain
    Immunohistochemical staining of mouse hippocampus and mouse cerebellum using Anti-CRF1/CRHR1 (extracellular) Antibody (#ACR-050), (1:600). A. CRF1 staining (green) in the cerebellum appears in cerebellar molecular layer (MOL). B. CRF1 staining (green) in the hippocampus appears in soma (horizontal arrows) and dendrites of pyramidal neurons (arrow). In both panels DAPI was used as the counterstain.
References
  1. Hollenstein, K. et al. (2013) Nature 499, 438.
  2. Rogers, J. et al. (2013) Mol. Psychiatry 18, 700.
Scientific background

Corticotrophin-releasing factor (CRF) is a peptide hormone and a key regulator of the stress response. Through activation of the hypothalamic–pituitary axis and its role as a neurotransmitter, CRF influences a wide range of physiological responses, including appetite control, cardiovascular regulation, glucose metabolism, immune function and behavior.

Corticotrophin-releasing factor receptor 1 (CRHR1, CRF1) is a member of the class B G-protein coupled receptors. CRHR1 is activated by CRF. It is expressed in brain areas including the hypothalamus, pituitary, amygdala and cortex. The core fold of the receptor features seven transmembrane helices (TM1-TM7). The connecting loops lack secondary structure apart from one extracellular loop- ECL1. In contrary to A Class GPCRs, CRF1 has a pronounced V-shape and a large cavity hypothesized to be the peptide binding site. The cytoplasmic half of TM3 and TM5 interacts with Gs. Small molecule antagonists of the receptor, such as CP-376395, act allosterically by maintaining the receptor in its inactive state at a site different from the peptide binding site. Interestingly, although CP-376395 is highly selective for CRHR1, all of the residues found to interact directly with this ligand are conserved between CRHR1 and CRHR21.

Interaction between childhood trauma and sequence variation in the CRHR1 gene that increase risk for affective disorders has been established by several studies. In Rhesus macaques, single nucleotide polymorphisms affecting exon 6 of CRHR1 gene influence anxious temperament (a phenotype that predicts the development of human anxiety and depressive disorder) and brain metabolism2.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 23/08/2020

Anti-CRF1/CRHR1 (extracellular) Antibody (#ACR-050) is a highly specific antibody directed against an extracellular epitope of the rat Corticotropin-releasing factor receptor 1. It can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope, and is thus ideal for detecting the protein in living cells. It has been designed to recognize CRFR1 from human, mouse, and rat samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Shipping and Ordering information