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- Peptide ENLSLTSNVSGLQC, corresponding to amino acid residues 31-44 of rat CRHR1 (Accession P35353). Extracellular, N-terminus.
- Western blot analysis of mouse brain membrane (lanes 1 and 4), rat brain membrane (lane 2 and 5) and rat cortex lysate (lane 3 and 6):1-3. Anti-CRF1/CRHR1 (extracellular) Antibody (#ACR-050), (1:200).
4-6. Anti-CRF1/CRHR1 (extracellular) Antibody, preincubated with the negative control antigen.Western blot analysis of human neuroblastoma (SH-SY5Y) (lanes 1 and 4), human brain astrocytoma (CCF-STTGI) (lanes 2 and 5) and rat pheochromocytoma (PC12) (lanes 3 and 6):1-3. Anti-CRF1/CRHR1 (extracellular) Antibody (#ACR-050), (1:200).
4-6. Anti-CRF1/CRHR1 (extracellular) Antibody, preincubated with the negative control antigen.
- Expression of Corticotropin-releasing factor receptor 1 in mouse brainImmunohistochemical staining of mouse hippocampus and mouse cerebellum using Anti-CRF1/CRHR1 (extracellular) Antibody (#ACR-050), (1:600). A. CRF1 staining (green) in the cerebellum appears in cerebellar molecular layer (MOL). B. CRF1 staining (green) in the hippocampus appears in soma (horizontal arrows) and dendrites of pyramidal neurons (arrow). In both panels DAPI was used as the counterstain.
Corticotrophin-releasing factor (CRF) is a peptide hormone and a key regulator of the stress response. Through activation of the hypothalamic–pituitary axis and its role as a neurotransmitter, CRF influences a wide range of physiological responses, including appetite control, cardiovascular regulation, glucose metabolism, immune function and behavior.
Corticotrophin-releasing factor receptor 1 (CRHR1, CRF1) is a member of the class B G-protein coupled receptors. CRHR1 is activated by CRF. It is expressed in brain areas including the hypothalamus, pituitary, amygdala and cortex. The core fold of the receptor features seven transmembrane helices (TM1-TM7). The connecting loops lack secondary structure apart from one extracellular loop- ECL1. In contrary to A Class GPCRs, CRF1 has a pronounced V-shape and a large cavity hypothesized to be the peptide binding site. The cytoplasmic half of TM3 and TM5 interacts with Gs. Small molecule antagonists of the receptor, such as CP-376395, act allosterically by maintaining the receptor in its inactive state at a site different from the peptide binding site. Interestingly, although CP-376395 is highly selective for CRHR1, all of the residues found to interact directly with this ligand are conserved between CRHR1 and CRHR21.
Interaction between childhood trauma and sequence variation in the CRHR1 gene that increase risk for affective disorders has been established by several studies. In Rhesus macaques, single nucleotide polymorphisms affecting exon 6 of CRHR1 gene influence anxious temperament (a phenotype that predicts the development of human anxiety and depressive disorder) and brain metabolism2.
Species reactivity key:
Anti-CRF1/CRHR1 (extracellular) Antibody (#ACR-050) is a highly specific antibody directed against an extracellular epitope of the rat Corticotropin-releasing factor receptor 1. It can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope, and is thus ideal for detecting the protein in living cells. It has been designed to recognize CRFR1 from human, mouse, and rat samples.