Anti-CRF1 (CRHR1) (extracellular)-FITC Antibody

Corticotropin-releasing factor receptor 1, CRFR1, Corticotropin-releasing hormone receptor 1, CRH-R1, CRH1
  • New
Cat #: ACR-050-F
Alternative Name Corticotropin-releasing factor receptor 1, CRFR1, Corticotropin-releasing hormone receptor 1, CRH-R1, CRH1
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
      • Peptide ENLSLTSNVSGLQC, corresponding to amino acid residues 31-44 of rat CRHR1 (Accession P35353). Extracellular, N-terminus.
    Accession (Uniprot) Number P35353
    Gene ID 58959
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse –12/14 amino acid residues identical; human – 11/14 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Label Fluorescein isothiocyanate (FITC).
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #ACR-050), and direct flow cytometry (labeled antibody).
    Applications: fc
    May also work in: ic, lci
      • Cell surface detection of CRF1 in live intact human MEG-01 megakaryoblastic leukemia cells:
        ___ Cells.
        ___ Cells + rabbit IgG isotype control-FITC.
        ___ Cells + Anti-CRF1 (CRHR1) (extracellular)-FITC Antibody (#ACR-050-F), (2.5 µg).
        Cell surface detection of CRF1 in live intact mouse P815 mastocytoma cells:
        ___ Cells.
        ___ Cells + rabbit IgG isotype control-FITC.
        ___ Cells + Anti-CRF1 (CRHR1) (extracellular)-FITC Antibody (#ACR-050-F), (2.5 µg).
      • Corticotrophin-releasing factor (CRF) is a peptide hormone and a key regulator of the stress response. Through activation of the hypothalamic–pituitary axis and its role as a neurotransmitter, CRF influences a wide range of physiological responses, including appetite control, cardiovascular regulation, glucose metabolism, immune function and behavior.

        Corticotrophin-releasing factor receptor 1 (CRHR1, CRF1) is a member of the class B G-protein coupled receptors. CRHR1 is activated by CRF. It is expressed in brain areas including the hypothalamus, pituitary, amygdala and cortex. The core fold of the receptor features seven transmembrane helices (TM1-TM7). The connecting loops lack secondary structure apart from one extracellular loop- ECL1. In contrary to A Class GPCRs, CRF1 has a pronounced V-shape and a large cavity hypothesized to be the peptide binding site. The cytoplasmic half of TM3 and TM5 interacts with Gs. Small molecule antagonists of the receptor, such as CP-376395, act allosterically by maintaining the receptor in its inactive state at a site different from the peptide binding site. Interestingly, although CP-376395 is highly selective for CRHR1, all of the residues found to interact directly with this ligand are conserved between CRHR1 and CRHR21.

        Interaction between childhood trauma and sequence variation in the CRHR1 gene that increase risk for affective disorders has been established by several studies. In Rhesus macaques, single nucleotide polymorphisms affecting exon 6 of CRHR1 gene influence anxious temperament (a phenotype that predicts the development of human anxiety and depressive disorder) and brain metabolism2.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat

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    Last update: 04/06/2019

    Anti-CRF1 (CRHR1) (extracellular) Antibody (#ACR-050) is a highly specific antibody directed against an extracellular epitope of the rat Corticotropin-releasing factor receptor 1. It can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope, and is thus ideal for detecting the protein in living cells. It has been designed to recognize CRFR1 from human, mouse, and rat samples.

    Anti-CRF1 (CRHR1) (extracellular)-FITC Antibody (#ACR-050-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

    For research purposes only, not for human use