Anti-CRF2/CRHR2 (extracellular) Antibody

Corticotropin-releasing factor receptor 2, CRF-R-2, CRF2 receptor beta isoform, Corticotropin-releasing hormone receptor 2, CRH receptor 2 variant B
    Cat #: ACR-052
    Alternative Name Corticotropin-releasing factor receptor 2, CRF-R-2, CRF2 receptor beta isoform, Corticotropin-releasing hormone receptor 2, CRH receptor 2 variant B
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    • Peptide (C)EELLLDGWGEPPDPE, corresponding to amino acid residues 20-34 of rat CRHR2 (Accession P47866). Extracellular, N-terminus.
    • Anti-CRF2/CRHR2 (extracellular) Antibody
    Accession (Uniprot) Number P47866
    Gene ID 64680
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse – 14/15 amino acid residues identical; human – 13/15 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ifc, wb
    May also work in: ic*, ih*, ip*, lci*
    Western blot
    • Anti-CRF2/CRHR2 (extracellular) Antibody
      Western blot analysis of mouse brain membrane (lanes 1 and 5), rat cerebellum (lanes 2 and 6), rat lung (lanes 3 and 7) and rat kidney (lanes 4 and 8) lysates:
      1-4. Anti-CRF2/CRHR2 (extracellular) Antibody (#ACR-052), (1:200).
      5-8. Anti-CRF2/CRHR2 (extracellular) Antibody, preincubated with the negative control antigen.
    • Anti-CRF2/CRHR2 (extracellular) Antibody
      Western blot analysis of MOLT-4 (lanes 1 and 5), CAKI-1 (lanes 2 and 6), MEG-O1 (lanes 3 and 7) and COLO-205 (lanes 4 and 8) cell line lysates:
      1-4. Anti-CRF2/CRHR2 (extracellular) Antibody (#ACR-052), (1:200).
      5-8. Anti-CRF2/CRHR2 (extracellular) Antibody, preincubated with the negative control antigen.
    Indirect flow cytometry
    • Anti-CRF2/CRHR2 (extracellular) Antibody
      Cell surface detection of Corticotropin-releasing factor receptor 2 in live intact HL-60 (human promyelocytic leukemia) cells:
      ___ Cells.
      ___ Cells + FITC-conjugated goat anti-rabbit antibody.
      ___ Cells + Anti-CRF2/CRHR2 (extracellular) Antibody (#ACR-052) (5 µg /5x105 cells) +FITC-conjugated goat anti-rabbit antibody.
    1. Pal, K. et al. (2010) J. Biol. Chem. 285, 40351.
    2. Fekete, E.M. and Zorrilla, E.P. (2007) Front. Neuroendocrinol. 28, 1.
    3. Bale, T.L. et al. (2003) Trends Cardiovasc. 13, 68.
    4. Yang, L.Z. et al. (2010) Eur. J. Pharmacol. 632, 1.
    5. Dieterle, T. et al. (2009) Hypertension 53, 739.
    6. Hillhouse, E.W. and Grammatopoulos, D.K. (2006) Endocr. Rev. 27, 260.
    Scientific background

    CRFR2 is a G-protein coupled receptor for CRF (corticotropin-releasing factor) and urocortins UCN1, UCN2, and UCN3. The receptor-ligand interaction causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and down-stream effectors, such as adenylate cyclase, leading to increased intracellular cAMP levels1.

    CRFR2 is expressed in the CNS and peripheral tissues including the heart, vasculature, skeletal muscle, and gastrointestinal tract, and it plays a central role in coordinating endocrine, autonomic, and behavioral responses to stress1. CRFR2 ligands, the UCN peptides, exert actions in central and peripheral tissues where they modulate cardiovascular, immune, gastrointestinal, and reproductive functions in response to stress2. UCNs can also affect tumor biology as a consequence of the CRFR2 role as a suppressor of vascularization3.

    The involvement of CRF/UCN family peptides and their receptors in diverse pathophysiological states has generated intense interest in developing therapeutic agents that selectively target the receptors. CRFR2-selective agonists have the potential to treat heart failure4, hypertension5 and other disorders.

    CRFR2 shares 68% amino acid sequence identity with another CRF receptor, CRFR1. Both of the receptors have a bipartite domain structure consisting of an N-terminal extracellular domain (ECD) of about 100–150 amino acids linked to a 7-transmembrane helical bundle domain embedded in the plasma membrane. Peptide binding spans the two domains, with the C-terminal portion of the peptide binding to the ECD to impart high affinity and the N-terminal portion binding to the 7-transmembrane domain to activate the receptor6.

    CRFR2 has three functional full-length isoforms, α, β, and γ, that differ only at the extreme N-terminus of the receptor. In humans, the CRFR2β isoform is expressed in the brain, and the CRFR2α isoform predominates in the periphery6.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Anti-CRF2/CRHR2 (extracellular) Antibody (#ACR-052) is a highly specific antibody directed against an epitope of the rat Corticotropin-releasing factor receptor 2. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize CRHR2 from rat, mouse, and human samples.

    For research purposes only, not for human use
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